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Titolo:
STRUCTURE DETERMINATION OF ISOMERIC HEXADEOXYNUCLEOTIDE PHOTOPRODUCTSBY HIGH-FIELD NMR AND FAST-ATOM-BOMBARDMENT TANDEM MASS-SPECTROMETRY
Autore:
VOLLMER D; ZHAO XD; TAYLOR JS; GROSS ML;
Indirizzi:
WASHINGTON UNIV,DEPT CHEM ST LOUIS MO 63130 WASHINGTON UNIV,DEPT CHEM ST LOUIS MO 63130 UNIV NEBRASKA,DEPT CHEM LINCOLN NE 68588
Titolo Testata:
International journal of mass spectrometry and ion processes
, volume: 165, anno: 1997,
pagine: 487 - 496
SICI:
0168-1176(1997)165:<487:SDOIHP>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
OLIGONUCLEOTIDES; DAMAGE;
Keywords:
T-RICH HEXADEOXYNUCLEOTIDES; PHOTOPRODUCTS; NMR; FAB; TANDEM MASS SPECTROMETRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
D. Vollmer et al., "STRUCTURE DETERMINATION OF ISOMERIC HEXADEOXYNUCLEOTIDE PHOTOPRODUCTSBY HIGH-FIELD NMR AND FAST-ATOM-BOMBARDMENT TANDEM MASS-SPECTROMETRY", International journal of mass spectrometry and ion processes, 165, 1997, pp. 487-496

Abstract

UV irradiation of a T-rich hexadeoxynucleotide, d(TATTAT), produced four photoproducts, which can be cleanly separated from starting material and each other by reverse-phase HPLC. The structures of the photoproducts were investigated by using 1D H-1-NMR, which allows the nature,but not the location, of the photoproduct between adjoining Ts or between T and A to be determined. Both fast atom bombardment (FAB) and matrix-assisted laser desorption ionization (MALDI) were able to confirmthe molecular weights of the starting material and the four photoproducts. Furthermore, the results of tandem mass spectrometry experiments, with a four-sector spectrometer, of the [M + H](+) ions of the various materials were consistent with structure and showed the location ofthe photoproduct in the d(TATTAT) molecule. All the isomers gave distinct product-ion spectra that are consistent with structure and sufficient for locating the site of the modification. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:55:58