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Titolo:
DOUBLE-STRANDED DNA-SEQUENCING BY TANDEM MASS-SPECTROMETRY
Autore:
MCLAFFERTY FW; AASERUD DJ; GUAN ZQ; LITTLE DP; KELLEHER NL;
Indirizzi:
CORNELL UNIV,BAKER LAB CHEM ITHACA NY 14853
Titolo Testata:
International journal of mass spectrometry and ion processes
, volume: 165, anno: 1997,
pagine: 457 - 466
SICI:
0168-1176(1997)165:<457:DDBTM>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTROSPRAY-IONIZATION; IONS; DISSOCIATION; PROTEINS; PRODUCTS;
Keywords:
ELECTROSPRAY; FOURIER-TRANSFORM MS; DNA; TANDEM; SEQUENCING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
F.W. Mclafferty et al., "DOUBLE-STRANDED DNA-SEQUENCING BY TANDEM MASS-SPECTROMETRY", International journal of mass spectrometry and ion processes, 165, 1997, pp. 457-466

Abstract

Electrospray ionization produces far more abundant molecular ions fordouble stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra ofeither individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3'-end heterogeneity. Published by Elsevier Science B.V.

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Documento generato il 09/08/20 alle ore 21:29:15