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Titolo:
HSP70-2 IS REQUIRED FOR DESYNAPSIS OF SYNAPTONEMAL COMPLEXES DURING MEIOTIC PROPHASE IN JUVENILE AND ADULT-MOUSE SPERMATOCYTES
Autore:
DIX DJ; ALLEN JW; COLLINS BW; POORMANALLEN P; MORI C; BLIZARD DR; BROWN PR; GOULDING EH; STRONG BD; EDDY EM;
Indirizzi:
US EPA,REPROD TOXICOL DIV,NATL HLTH & ENVIRONM EFFECTS RES LAB RES TRIANGLE PK NC 27711 US EPA,DIV ENVIRONM CARCINOGENESIS,NATL HLTH & ENVIRONM EFFECTS RES LAB RES TRIANGLE PK NC 27711 GLAXO WELLCOME INC,DIV MED SAFETY EVALUAT RES TRIANGLE PK NC 27709 KYOTO UNIV,FAC MED,DEPT ANAT & DEV BIOL KYOTO 60601 JAPAN NIEHS,REPROD & DEV TOXICOL LAB,NIH RES TRIANGLE PK NC 27709
Titolo Testata:
Development
fascicolo: 22, volume: 124, anno: 1997,
pagine: 4595 - 4603
SICI:
0950-1991(1997)124:22<4595:HIRFDO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
DEVELOPMENTALLY-REGULATED EXPRESSION; RAD51 RECOMBINATION PROTEIN; HEAT-SHOCK PROTEINS; DNA MISMATCH REPAIR; SPERMATOGENIC CELLS; CDC2 PROTEIN; GERM-CELL; NUCLEOTIDE-SEQUENCE; FIBROUS SHEATH; GENE FAMILY;
Keywords:
HEAT SHOCK PROTEIN; HSP70; MEIOSIS; SPERMATOGENESIS; SYNAPTONEMAL COMPLEX; MOUSE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
D.J. Dix et al., "HSP70-2 IS REQUIRED FOR DESYNAPSIS OF SYNAPTONEMAL COMPLEXES DURING MEIOTIC PROPHASE IN JUVENILE AND ADULT-MOUSE SPERMATOCYTES", Development, 124(22), 1997, pp. 4595-4603

Abstract

Spermatogenic cells synthesize a unique 70-kDa heat shock protein (HSP70-2) during prophase of meiosis I, and targeted disruption of the Hsp 70-2 gene has shown that this protein is required for spermatogenic cell differentiation in adult mice, HSP70-2 is associated with synaptonemal complexes formed between paired homologous chromosomes during meiotic prephase, The present study focuses on the nearly synchronous first wave of spermatogenesis in 12- to 28-day old juvenile mice to determine more precisely when HSP70-2 is required and what meiotic processes are affected by its absence. Spermatogenesis in homozygous mutant mice (Hsp70-2(-/-)) proceeded normally until day 15 when increasing numbers of pachytene spermatocytes became apoptotic and differentiation of cells beyond the pachytene stage began to falter, Synaptonemal complexes assembled in Hsp 70-2(-/-) mice and spermatocytes developed through the final pachytene substage, However, synaptonemal complexes failed to desynapse and normal diplotene spermatocytes were not observed, Metaphase spermatocytes were not seen in tissue sections from testes ofHsp70-2(-/-) mice, and expression of mRNAs and antigens characteristic of late pachytene spermatocytes (e.g., cyclin Al) and development ofspermatids did not occur. Thus, HSP70-2 is required for synaptonemal complex desynapsis, and its absence severely impairs the transition ofspermatogenic cells through the late meiotic stages and results in apoptosis beginning with the first wave of germ cell development in juvenile mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 19:04:26