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Titolo:
CHRONIC HYPOXIA INDUCES PROLIFERATION OF CULTURED MESANGIAL CELLS - ROLE OF CALCIUM AND PROTEIN-KINASE-C
Autore:
SAHAI A; MEI CL; PATTISON TA; TANNEN RL;
Indirizzi:
UNIV COLORADO,HLTH SCI CTR,DIV RENAL DIS & HYPERTENS,CAMPUS BOX C281,4200 E 9TH AVE DENVER CO 80262 UNIV SO CALIF,SCH MED,DEPT MED LOS ANGELES CA 90033 UNIV PENN,SCH MED PHILADELPHIA PA 19104
Titolo Testata:
American journal of physiology. Renal, fluid and electrolyte physiology
fascicolo: 6, volume: 42, anno: 1997,
pagine: 954 - 960
SICI:
0363-6127(1997)42:6<954:CHIPOC>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ENDOTHELIAL-CELLS; SMOOTH-MUSCLE CELLS; LOW-OXYGEN TENSION; RENAL ISCHEMIA; DIFFERENTIATION; FIBROBLASTS; ACTIVATION; DISEASE; INJURY; GROWTH;
Keywords:
MESANGIAL CELL GROWTH; SIGNAL TRANSDUCTION;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
A. Sahai et al., "CHRONIC HYPOXIA INDUCES PROLIFERATION OF CULTURED MESANGIAL CELLS - ROLE OF CALCIUM AND PROTEIN-KINASE-C", American journal of physiology. Renal, fluid and electrolyte physiology, 42(6), 1997, pp. 954-960

Abstract

The effect of hypoxia on the proliferation of cultured rat mesangial cells was examined. To evaluate the underlying signaling mechanisms, the roles of intracellular calcium ([Ca2+](i)) and protein kinase C (PKC) were determined. Quiescent cultures were exposed to hypoxia (3% O-2) or normoxia (18% O-2), and [H-3]thymidine incorporation, cell number, [Ca2+](i), and PKC were assessed. Mesangial cells exposed to 28 h ofhypoxia exhibited a significant increase in [H-3]thymidine incorporation followed by a significant increase in cell number at 72 h in comparison with respective normoxic controls. Hypoxia induced a biphasic activation of PKC, reflected by translocation of the enzyme activity from cytosol to membrane at 1 h, a return to baseline at 4 and 8 h, with subsequent reactivation from 16 to 48 h. In addition, hypoxia-induced proliferation was prevented by a PKC inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7). Cells exposed to hypoxia produced progressive increases in resting [Ca2+](i) from 15 to 60 min which remain sustained up to 24 h of examination. Verapamil significantly prevented the hypoxia-induced proliferation, and both verapamil treatment and incubations in a calcium-free medium for 1 h blocked the hypoxia-inducedstimulation of[Ca2+](i) as well as PKC. These results provide the first in vitro evidence that chronic hypoxia induces proliferation of cultured glomerular mesangial cells, which is mediated by the stimulationof [Ca2+](i) and the subsequent activation of PKC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/10/20 alle ore 05:41:56