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Titolo:
COMPARISON OF 2 METHODS OF STAINING APOPTOTIC CELLS OF LEUKEMIA-CELL LINES - TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE AND DNA-POLYMERASE-I REACTIONS
Autore:
YAMADORI I; YOSHINO T; KONDO E; CAO L; AKAGI T; MATSUO Y; MINOWADA J;
Indirizzi:
OKAYAMA UNIV,SCH MED,DEPT PATHOL,2-5-1 SHIKATA CHO OKAYAMA 700 JAPAN HAYASHIBARA BIOCHEM LABS INC,FUJISAKI CELL CTR OKAYAMA JAPAN
Titolo Testata:
The Journal of histochemistry and cytochemistry
fascicolo: 1, volume: 46, anno: 1998,
pagine: 85 - 90
SICI:
0022-1554(1998)46:1<85:CO2MOS>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
NON-HODGKINS-LYMPHOMAS; BCL-2 PROTEIN; FRAGMENTATION; DEATH; INSITU; FAS; POLY(ADP-RIBOSYL)ATION; EXPRESSION; NECROSIS; SECTIONS;
Keywords:
APOPTOSIS; LEUKEMIA CELL LINE; DNA CLEAVAGE; DNA POLYMERASE I; TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
I. Yamadori et al., "COMPARISON OF 2 METHODS OF STAINING APOPTOTIC CELLS OF LEUKEMIA-CELL LINES - TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE AND DNA-POLYMERASE-I REACTIONS", The Journal of histochemistry and cytochemistry, 46(1), 1998, pp. 85-90

Abstract

We compared two methods to stain apoptotic cells, one using terminal deoxynucleotidyl transferase (TDT), the other DNA polymerase I, using leukemia cell lines treated with anti-fas monoclonal antibody (MAb). Both TDT and polymerase I strongly reacted with fragmented nuclei of apoptotic MOLT-16 and Jurkat cells, but only polymerase strongly reactedwith nonfragmented nuclei of early apoptotic cells. Anti-Fas MAb-treated MOLT-4 cells showed morphological changes corresponding to early apoptosis and were strongly positive for polymerase I only. MOLT-16 andJurkat cells treated with anti-fas MAb and inhibitors of endonucleaseand poly(ADP-ribose) polymerase showed the morphology of early apoptosis but were not strongly stained by TDT. Because DNA polymerase I hasnick-translation activity, it is possible that DNA polymerase I reaction is positive in early apoptotic cells by detecting single-strand DNA cleavage, which occurs before extensive oligonucleosomal DNA cleavage and [ate morphological changes of apoptosis in leukemia cell lines. Although TDT is widely used to stain apoptotic cells, DNA polymerase Imay be more applicable in special cases of apoptosis, in which cells undergo single-strand rather than double-strand DNA breaks. However, the procedure has limitations, such as the necessity to use cell smearsfor comparison with the TDT reaction.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 15:31:00