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Titolo:
IS THE UPTAKE OF PEFLOXACIN IN HUMAN BLOOD MONOCYTES A SIMPLE DIFFUSION PROCESS
Autore:
MEMIN E; PANTEIX G; REVOL A;
Indirizzi:
CTR HOSP LYON SUD,SERV BIOCHIM,BAT 3B,SECTEUR J COURMONT F-69310 PIERRE BENITE FRANCE INST PASTEUR,LAB MYCOBACTERIES F-69363 LYON 07 FRANCE
Titolo Testata:
Journal of antimicrobial chemotherapy
fascicolo: 5, volume: 38, anno: 1996,
pagine: 787 - 798
SICI:
0305-7453(1996)38:5<787:ITUOPI>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POLYMORPHONUCLEAR LEUKOCYTES; MACROPHAGES; LOCALIZATION; ACCUMULATION; ANTIBIOTICS; ENTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
17
Recensione:
Indirizzi per estratti:
Citazione:
E. Memin et al., "IS THE UPTAKE OF PEFLOXACIN IN HUMAN BLOOD MONOCYTES A SIMPLE DIFFUSION PROCESS", Journal of antimicrobial chemotherapy, 38(5), 1996, pp. 787-798

Abstract

Effective therapy of infections caused by facultative intracellular micro-organisms, which persist after ingestion by mononuclear phagocytic cells, requires antibiotics which inactivate these intraphagocytic bacteria. A previous study demonstrated that intracellular concentrations of normethylpefloxacin, C-i, were the result of simple diffusion and an active efflux involving organic anion transporters. In our work, we studied pefloxacin transport in human monocytes. A kinetic approachwas followed to establish the processes involved. Extracellular concentrations, C-e, corresponding to in-vivo pharmacological levels of thedrug were used. Uptake of the antibiotic was assessed principally by HPLC. Pefloxacin was accumulated by the cells (C-i/C-e = 3) The uptakewas rapid, non saturable, reversible and temperature dependent. At 4 degrees C, the C-i/C-e was equal to 1. At high temperatures, an activeand initial process appeared that was not visible at lower temperatures and disappeared in presence of energetic metabolism inhibitor: At 42 degrees C, NaCN and 2.4 DNP (1 mM) reduced the initial transport butthey had no significant effect on the subsequent curve.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:31:43