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Titolo:
EXPRESSION OF NEURAL CELL-ADHESION MOLECULE L1 IN HUMAN LUNG-CANCER CELL-LINES
Autore:
KATAYAMA M; IWAMATSU A; MASUTANI H; FURUKE K; TAKEDA K; WADA H; MASUDA T; ISHII K;
Indirizzi:
KYOTO UNIV,INST VIRUS RES,SAKYO KU,SHOGOIN KAWAHARA CHO KYOTO 60601 JAPAN KYOTO UNIV,INST VIRUS RES,SAKYO KU KYOTO 60601 JAPAN KYOTO UNIV,CHEST DIS RES INST,SAKYO KU KYOTO 60601 JAPAN KIRIN BREWERY CO LTD,CENT LABS KEY TECHNOL,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN KYOTO UNIV,FAC MED,DEPT IMMUNOBIOL,SAKYO KU KYOTO 606 JAPAN
Titolo Testata:
Cell structure and function
fascicolo: 5, volume: 22, anno: 1997,
pagine: 511 - 516
SICI:
0386-7196(1997)22:5<511:EONCML>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MOUSE MONOCLONAL-ANTIBODIES; NERVE-LIKE CELLS; HUMAN NEUROBLASTOMA; SURFACE ANTIGENS; BLASTOMA; DIFFERENTIATION; VARIANTS; PROTEINS; INVITRO; BRAIN;
Keywords:
CELL ADHESION MOLECULE L1; CARCINOMA; LUNG CANCER; NEUROBLASTOMA; MONOCLONAL ANTIBODY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
M. Katayama et al., "EXPRESSION OF NEURAL CELL-ADHESION MOLECULE L1 IN HUMAN LUNG-CANCER CELL-LINES", Cell structure and function, 22(5), 1997, pp. 511-516

Abstract

To investigate neuron-specific antigens, hybridoma cells were produced between mouse spleen cells immunized with human neuroblastoma cells (IMR-32) and mouse myeloma cells. 247 hybridoma clones were harvested and one of them was further cultured for recloning. Eventually, one hybridoma clone was obtained and its antibody was designated N-A8. The characteristics of this antibody were determined by immunostaining and flow cytometry. First, the antibody recognized the surface antigens ofIMR-32 cells. Second, unexpectedly, N-A8 was reactive not only with human neuroblastoma cell lines but also with human lung cancer cell lines. As analyzed by immunoprecipitation method and SDS-PAGE, the molecular size of the antigen recognized by N-A8 was 210 kDa. The antigen was then purified by affinity chromatography and identified as neural adhesion molecule L1 by amino acid sequence analysis. By the present investigation, it was clearly demonstrated that LI is expressed in human lung cancer cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 08:38:55