Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
AUTOMATED ONLINE DIALYSIS, TRACE ENRICHMENT AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY INHIBITION OF INTERACTION WITH THE DIALYSIS MEMBRANEAND DISRUPTION OF PROTEIN-BINDING IN THE DETERMINATION OF CLOZAPINE IN HUMAN PLASMA
Autore:
JOHANSEN K; KROGH M; RASMUSSEN KE;
Indirizzi:
UNIV OSLO,SCH PHARM,POB 1068 BLINDERN N-0316 OSLO NORWAY UNIV OSLO,SCH PHARM N-0316 OSLO NORWAY
Titolo Testata:
Journal of chromatography B. Biomedical sciences and applications
fascicolo: 1-2, volume: 690, anno: 1997,
pagine: 223 - 231
SICI:
0378-4347(1997)690:1-2<223:AODTEA>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; ONLINE DIALYSIS; SAMPLE PREPARATION; WHOLE-BLOOD; ULTRAVIOLET DETECTION; GAS-CHROMATOGRAPHY; HUMAN SERUM; DESMETHYLCLOZAPINE; OXYTETRACYCLINE; BENZODIAZEPINES;
Keywords:
PROTEIN BINDING; MEMBRANE INTERACTION; CLOZAPINE; N-DESMETHYLCLOZAPINE; PROTEINS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
K. Johansen et al., "AUTOMATED ONLINE DIALYSIS, TRACE ENRICHMENT AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY INHIBITION OF INTERACTION WITH THE DIALYSIS MEMBRANEAND DISRUPTION OF PROTEIN-BINDING IN THE DETERMINATION OF CLOZAPINE IN HUMAN PLASMA", Journal of chromatography B. Biomedical sciences and applications, 690(1-2), 1997, pp. 223-231

Abstract

Problems related to interaction of drugs with the dialysis membrane and to protein binding must be overcome in order to develop automated methods for drug analysis based on on-line dialysis, trace enrichment and HPLC. In order to study these problems, clozapine and its active metabolite N-desmethylclozapine were chosen as model compounds because they were found to interact with the dialysis membrane, and clozapine is highly protein bound. Addition of a cationic surfactant, dodecylethyldimethyl ammonium bromide, to the donor solution and to the plasma samples was found to inhibit interaction of the drugs with surfaces. Theprotein binding in plasma was disrupted prior to dialysis by loweringthe pH with hydrochloric acid and the plasma proteins were solubilised with glycerol. The results obtained were used to develop a fully automated method for the determination of clozapine and N-desmethylclozapine in human plasma. More than 100 samples could be analysed within 24h. The limit of detection in human plasma was 0.050 mu mol/l for clozapine and 0.055 mu mol/l for N-desmethylclozapine. Linearity was foundfor drug concentrations between 0.25-3 mu mol/l. The relative standard deviations were between 1.2-6.7% and the method was applicable for therapeutic drug monitoring.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/01/20 alle ore 01:10:18