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Titolo:
DETERMINATION OF CLOZAPINE AND ITS MAJOR METABOLITES IN HUMAN PLASMA AND RED-BLOOD-CELLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET ABSORBENCY DETECTION
Autore:
GUITTON C; KINOWSKI JM; AZNAR R; BRESSOLLE F;
Indirizzi:
HOP CAREMEAU,LAB PHARMACOCINET F-30900 NIMES FRANCE HOP CAREMEAU,LAB PHARMACOCINET F-30900 NIMES FRANCE FAC PHARM MONTPELLIER,LAB PHARMACOCINET F-34060 MONTPELLIER 01 FRANCE
Titolo Testata:
Journal of chromatography B. Biomedical sciences and applications
fascicolo: 1-2, volume: 690, anno: 1997,
pagine: 211 - 222
SICI:
0378-4347(1997)690:1-2<211:DOCAIM>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
SOLID-PHASE EXTRACTION; HUMAN-SERUM; GAS-CHROMATOGRAPHY; N-OXIDE; DESMETHYLCLOZAPINE; SCHIZOPHRENIA;
Keywords:
CLOZAPINE; N-DESMETHYLCLOZAPINE; CLOZAPINE N-OXIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
C. Guitton et al., "DETERMINATION OF CLOZAPINE AND ITS MAJOR METABOLITES IN HUMAN PLASMA AND RED-BLOOD-CELLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET ABSORBENCY DETECTION", Journal of chromatography B. Biomedical sciences and applications, 690(1-2), 1997, pp. 211-222

Abstract

An isocratic high-performance liquid chromatographic (HPLC) method with UV absorbance detection is described for the quantification of clozapine '-methyl)piperazino-5H-dibenzo[b,e]-1,4-diazepine) and its two major metabolites in plasma and red blood cells (RBCs). The method involves sample clean-up by liquid-liquid extraction with ethyl acetate. The organic phase was back-extracted with 0.1 M hydrochloric acid. Loxapine served as the internal standard. The analytes were separated by HPLC on a Kromasil Ultrabase C-18 analytical column (5 mu m particle size; 250X4.6 mm I.D.) using acetonitrile-phosphate buffer pH 7.0 (48:52, v/v) as eluent and were measured by UV absorbance detection at 254 nm. The limits of quantitation were 20 ng/ml for clozapine and N-desmethylclozapine and 30 ng/ml for clozapine N-oxide. Recovery from plasma or RBCs proved to be higher than 62%. Precision, expressed as % C.V., was in the range 0.6-15%. Accuracy ranged from 96 to 105%. The method's ability to quantify clozapine;md two major metabolites simultaneously with precision, accuracy and sensitivity makes it useful in therapeutic drug monitoring.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 17:51:42