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Titolo:
TRANSFORMATION BY WNT FAMILY PROTEINS CORRELATES WITH REGULATION OF BETA-CATENIN
Autore:
SHIMIZU H; JULIUS MA; GIARRE M; ZHENG ZL; BROWN AMC; KITAJEWSKI J;
Indirizzi:
COLUMBIA UNIV,COLL PHYS & SURG,DEPT PATHOL,630 W 168TH ST NEW YORK NY10032 COLUMBIA UNIV,COLL PHYS & SURG,DEPT PATHOL NEW YORK NY 10032 COLUMBIA UNIV,COLL PHYS & SURG,CTR REPROD SCI NEW YORK NY 10032 COLUMBIA UNIV,COLL MED,STRANG CORNELL CANC RES LAB NEW YORK NY 10021 COLUMBIA UNIV,COLL MED,DEPT CELL BIOL & ANAT NEW YORK NY 10021
Titolo Testata:
Cell growth & differentiation
fascicolo: 12, volume: 8, anno: 1997,
pagine: 1349 - 1358
SICI:
1044-9523(1997)8:12<1349:TBWFPC>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMARY EPITHELIAL-CELLS; POLARITY GENE ARMADILLO; PROTO-ONCOGENE INT-1; PROVIRAL ACTIVATION; MOUSE DEVELOPMENT; WINGLESS PROTEIN; NEURAL-TUBE; PC12 CELLS; EXPRESSION; DROSOPHILA;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
62
Recensione:
Indirizzi per estratti:
Citazione:
H. Shimizu et al., "TRANSFORMATION BY WNT FAMILY PROTEINS CORRELATES WITH REGULATION OF BETA-CATENIN", Cell growth & differentiation, 8(12), 1997, pp. 1349-1358

Abstract

Several members of the Wnt family of secreted factors are strongly implicated as regulators of mammary cell growth and differentiation. To investigate Wnt signaling in mammary cells, we have assessed the abilities of 10 different Wnt genes to cause transformation of C57MG mammary epithelial cells and in parallel studied their effects on beta-catenin, a component of the Wnt-1 signaling pathway. Autocrine transformingpotential was tested by expression of Wnt proteins in C57MG cells, and paracrine effects were evaluated by coculture of C57MG cells with fibroblasts secreting different Wnt proteins. Western blotting confirmedthe expression of each Wnt protein in the relevant cell lines. Activities of the 10 Wnts tested were divisible into three groups. Wnt-1, Wnt-2, Wnt-3, and Wnt-3a induced strong transformation and an elongated refractile cell morphology. Wnt-6 and Wnt-7a produced weak morphological changes. Wnt-4, Wnt-5a, Wnt-5b, and Wnt-7b had no effect at all on C57MG morphology. Analysis of beta-catenin levels showed that the transforming Wnts induced accumulation of cytosolic beta-catenin, whereas nontransforming Wnts did not. These results demonstrate that several Wnt family members are capable of elevating beta-catenin levels and suggest that their signaling pathways share intracellular signaling components. The correlation between increased cytosolic beta-catenin levelsand C57MG transformation supports a role for beta-catenin in transformation of these cells. These data also imply the existence of receptors that respond to certain Wnt proteins but not to others.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:23:16