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Titolo:
STRUCTURAL AND FUNCTIONAL FEATURES OF A SPECIFIC NUCLEOSOME CONTAINING A RECOGNITION ELEMENT FOR THE THYROID-HORMONE RECEPTOR
Autore:
WONG JM; LI Q; LEVI BZ; SHI YB; WOLFFE AP;
Indirizzi:
NICHHD,MOL EMBRYOL LAB,NIH,BLDG 18T,ROOM 106 BETHESDA MD 20892 NICHHD,MOL EMBRYOL LAB,NIH BETHESDA MD 20892
Titolo Testata:
EMBO journal
fascicolo: 23, volume: 16, anno: 1997,
pagine: 7130 - 7145
SICI:
0261-4189(1997)16:23<7130:SAFFOA>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
RIBOSOMAL-RNA GENE; GLUCOCORTICOID RESPONSE ELEMENT; TRANSCRIPTION FACTOR ACCESS; TUMOR VIRUS PROMOTER; LINKER HISTONES; POSITIONED NUCLEOSOMES; GLOBULAR DOMAIN; CORE HISTONES; BINDING-SITE; IN-VIVO;
Keywords:
CHROMATIN STRUCTURE; LINKER HISTONES; NUCLEAR RECEPTORS; NUCLEOSOME POSITIONING; TRANSCRIPTIONAL REGULATION;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
80
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Wong et al., "STRUCTURAL AND FUNCTIONAL FEATURES OF A SPECIFIC NUCLEOSOME CONTAINING A RECOGNITION ELEMENT FOR THE THYROID-HORMONE RECEPTOR", EMBO journal, 16(23), 1997, pp. 7130-7145

Abstract

The Xenopus thyroid hormone receptor beta A (TR beta A) gene containsan important thyroid hormone response element (TRE) that is assembledinto a positioned nucleosome. We determine the translational positionof the nucleosome containing the TRE and the rotational positioning of the double helix with respect to the histone surface, Histone H1 is incorporated into the nucleosome leading to an asymmetric protection to micrococcal nuclease cleavage of linker DNA relative to the nucleosome core. Histone H1 association is without significant consequence forthe binding of the heterodimer of thyroid hormone receptor and 9-cis retinoic acid receptor (TR/RXR) to nucleosomal DNA ill vitro, or for the regulation of TR beta A gene transcription following microinjectioninto the oocyte nucleus, Small alterations of 3 and 6 bp in the translational positioning of the TRE in chromatin are also without effect on the transcriptional activity of the TR beta A gene, whereas a small change in the rotational position of the TRE (3 bp) relative to the histone surface significantly reduces the binding of TR/RXR to the nucleosome and decreases transcriptional activation directed by TR/RXR. Ourresults indicate that the specific architecture of the nucleosome containing the TRE may have regulatory significance for expression of theTR beta A gene.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:08:59