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Titolo:
CLONING AND CHARACTERIZATION OF CNEMDR1 - A CRYPTOCOCCUS-NEOFORMANS GENE ENCODING A PROTEIN RELATED TO MULTIDRUG-RESISTANCE PROTEINS
Autore:
THORNEWELL SJ; PEERY RB; SKATRUD PL;
Indirizzi:
ELI LILLY & CO,LILLY RES LABS,LILLY CORP CTR,INFECT DIS RES,DC 0428 INDIANAPOLIS IN 46285 ELI LILLY & CO,LILLY RES LABS,LILLY CORP CTR,INFECT DIS RES INDIANAPOLIS IN 46285
Titolo Testata:
Gene
fascicolo: 1-2, volume: 201, anno: 1997,
pagine: 21 - 29
SICI:
0378-1119(1997)201:1-2<21:CACOC->2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-ALBICANS; VIRULENCE; DNA; EXPORT; CELLS; FUNGI; TOOL;
Keywords:
ABC TRANSPORTER PROTEIN; FUNGI; ENCAPSULATED YEAST; OPPORTUNISTIC PATHOGEN; KARYOTYPE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
S.J. Thornewell et al., "CLONING AND CHARACTERIZATION OF CNEMDR1 - A CRYPTOCOCCUS-NEOFORMANS GENE ENCODING A PROTEIN RELATED TO MULTIDRUG-RESISTANCE PROTEINS", Gene, 201(1-2), 1997, pp. 21-29

Abstract

CneMDR1, a gene encoding a protein related to several eukaryotic multidrug resistance (MDR) proteins, was identified, cloned, and characterized from a clinical isolate of Cryptococcus neoformans (Cn) (strain M1-106). Polymerase chain reaction (PCR) amplification of a DNA region encompassing conserved motifs of other MDR-like proteins was initiallyused to identify and clone CneMDR1. Analysis of the corresponding cDNA revealed an open reading frame punctuated by 16 introns. CneMDR1 encoded a protein (CNEMDR1) containing 1408 amino acids (aa) with a predicted mass of similar to 152 kDa. Protein structure predictions suggested the presence of two putative 6-transmembrane (TM) domains as well as two ATP-binding domains, structural characteristics typical of ATP-binding cassette (ABC) proteins. Members of this superfamily, which include MDR proteins, are frequently involved in active transport of a variety of substrates across the cell membrane. Pulsed-field gel electrophoresis revealed the presence of 12 chromosomal bands in this clinical isolate of Cn. CneMDR1 was detected by hybridization on chromosome IV. High-stringency hybridization detected only one MDR-like gene. However, a second MDR-like gene (CneMDR2) was discovered during reverse transcriptase-PCR (RT-PCR) amplification using cDNA. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 05:29:20