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Titolo:
OPTIMUM COMBINATION OF REVERSED-PHASE COLUMN TYPE AND MOBILE-PHASE COMPOSITION FOR GRADIENT ELUTION ION-PAIR CHROMATOGRAPHY OF AMINO-ACIDS
Autore:
YOKOYAMA Y; AMAKI T; HORIKOSHI S; SATO H;
Indirizzi:
YOKOHAMA NATL UNIV,FAC ENGN,ANALYT CHEM LAB,156 TOKIWADAI YOKOHAMA KANAGAWA 240 JAPAN
Titolo Testata:
Analytical sciences
fascicolo: 6, volume: 13, anno: 1997,
pagine: 963 - 967
SICI:
0910-6340(1997)13:6<963:OCORCT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; ORTHO-PHTHALALDEHYDE; PICOMOLE RANGE; DERIVATIZATION; SEPARATION;
Keywords:
ION-PAIR HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; GRADIENT ELUTION; SODIUM DODECYL-SULFATE; SODIUM OCTANESULFONATE; AMINO ACID; ORGANIC CATION;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
14
Recensione:
Indirizzi per estratti:
Citazione:
Y. Yokoyama et al., "OPTIMUM COMBINATION OF REVERSED-PHASE COLUMN TYPE AND MOBILE-PHASE COMPOSITION FOR GRADIENT ELUTION ION-PAIR CHROMATOGRAPHY OF AMINO-ACIDS", Analytical sciences, 13(6), 1997, pp. 963-967

Abstract

An optimum combination of reversed-phase column type and eluent composition was selected for the practical separation of amino acids by gradient elution ion-pair chromatography. A butyl-silica (C4) column witha concentration gradient elution between 5 mM SDS-10 mM HClO4 solution and acetonitrile provided the best resolution for amino acids. The C4 column was most adequate for reducing the chromatographic cycle timeincluding the column regeneration. The use of 5 mM SDS as ion-pairingagent in relatively low concentration is economical and practical forreplicate and routine analyses. The developed HPLC system is applicable to the analysis of organic cations at low cost.

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Documento generato il 12/07/20 alle ore 12:59:54