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Titolo:
INFLUENCE OF VANADATE ON GLYCOLYSIS, INTRACELLULAR SODIUM, AND PH IN PERFUSED RAT HEARTS
Autore:
GERALDES CFGC; CASTRO MMCA; SHERRY AD; RAMASAMY R;
Indirizzi:
UNIV COIMBRA,DEPT BIOCHEM,FAC SCI & TECHNOL,POB 3126 P-3000 COIMBRA PORTUGAL UNIV TEXAS,DEPT CHEM RICHARDSON TX 75083 UNIV TEXAS,SW MED CTR,DEPT RADIOL,MARY NELL & RALPH B ROGERS MAGNET RESONANCE CTR DALLAS TX 75235 UNIV TEXAS,SW MED CTR,DEPT INTERNAL MED,DIV CARDIOL DALLAS TX 75235 UNIV CALIF DAVIS,DIV CARDIOVASC MED DAVIS CA 95616
Titolo Testata:
Molecular and cellular biochemistry
fascicolo: 1-2, volume: 170, anno: 1997,
pagine: 53 - 63
SICI:
0300-8177(1997)170:1-2<53:IOVOGI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
DIABETIC RATS; INSULIN-RECEPTOR; BLOOD-GLUCOSE; VANADIUM; (NA,K)-ATPASE; MUSCLE; INHIBITION; MYOCARDIUM; PHOSPHORYLATION; DISSOCIATION;
Keywords:
VANADATE EFFECTS; GLYCOLYSIS; INTRACELLULAR SODIUM; NMR; PERFUSED RAT HEARTS; ISCHEMIA-REPERFUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
58
Recensione:
Indirizzi per estratti:
Citazione:
C.F.G.C. Geraldes et al., "INFLUENCE OF VANADATE ON GLYCOLYSIS, INTRACELLULAR SODIUM, AND PH IN PERFUSED RAT HEARTS", Molecular and cellular biochemistry, 170(1-2), 1997, pp. 53-63

Abstract

Vanadium compounds have been shown to cause a variety of biological and metabolic effects including inhibition of certain enzymes, alteration of contractile function, and as an insulin like regulator of glucose metabolism. However, the influence of vanadium on metabolic and ionic changes in hearts remains to be understood. In this study we have examined the influence of vanadate on glucose metabolism and sodium transport in isolated perfused rat hearts. Hearts were perfused with 10 mMglucose and varying vanadate concentrations (0.7-100 mu M) while changes in high energy phosphates (ATP and phosphocreatine (PCr)), intracellular pH, and intracellular sodium were monitored using P-31 and Na-23 NMR spectroscopy. Tissue lactate, glycogen, and (Na+, K+)-ATPase activity were also measured using biochemical assays. Under baseline conditions, vanadate increased tissue glycogen levels two fold and reduced(Na+, K+)-ATPase activity. Significant decreases in ATP and PCr were observed in the presence of vanadate, with little change in intracellular pH. These changes under baseline conditions were less severe when the hearts were perfused with glucose, palmitate and beta-hydroxybutyrate. During ischemia vanadate did not limit the rise in intracellular sodium, but slowed sodium recovery on reperfusion. The presence of vanadate during ischemia resulted in attenuation of acidosis, and reducedlactate accumulation. Reperfusion in the presence of vanadate resulted in a slower ATP recovery, while intracellular pH and PCr recovery was not affected. These results indicate that vanadate alters glucose utilization and (Na+, K+)-ATPase activity and thereby influences the response of the myocardium to an ischemic insult.

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Documento generato il 27/11/20 alle ore 07:02:59