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Titolo:
CYTOKINE MESSENGER-RNA EXPRESSION IN INTESTINAL TISSUE OF INTERLEUKIN-2 DEFICIENT MICE WITH BOWEL INFLAMMATION
Autore:
AUTENRIETH IB; BUCHELER N; BOHN E; HEINZE G; HORAK I;
Indirizzi:
UNIV MUNICH,MAX V PETTENKOFER INST HYG & MED MIKROBIOL,PETTENKOFERSTR9A D-80336 MUNICH GERMANY UNIV WURZBURG,INST VIROL & IMMUNOBIOL D-8700 WURZBURG GERMANY
Titolo Testata:
Gut
fascicolo: 6, volume: 41, anno: 1997,
pagine: 793 - 800
SICI:
0017-5749(1997)41:6<793:CMEIIT>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CD4(+) T-CELLS; MESSENGER-RNA EXPRESSION; ULCERATIVE-COLITIS; YERSINIA-ENTEROCOLITICA; GENE-EXPRESSION; RECEPTOR ANTAGONIST; EPITHELIAL-CELLS; CROHNS-DISEASE; TGF-BETA; PROLIFERATION;
Keywords:
CYTOKINE; MESSENGER-RNA EXPRESSION; INTERLEUKIN-2 DEFICIENT MICE; BOWEL INFLAMMATION;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
57
Recensione:
Indirizzi per estratti:
Citazione:
I.B. Autenrieth et al., "CYTOKINE MESSENGER-RNA EXPRESSION IN INTESTINAL TISSUE OF INTERLEUKIN-2 DEFICIENT MICE WITH BOWEL INFLAMMATION", Gut, 41(6), 1997, pp. 793-800

Abstract

Background-Mice deficient in interleukin-2 (IL-2) develop inflammatory bowel disease resembling ulcerative colitis in humans. Recent studies provided evidence that alpha beta T cells, particularly CD4 T cells,rather than B cells, are involved in the pathogenesis of bowel inflammation of IL-2 deficient mice. Aim-To analyse the pattern of expression of cytokine mRNA in intestinal tissue of normal and IL-2 deficient mice. Methods-Expression of beta-actin, IL-1 alpha, IL-1 beta, IL-6, IL-10, tumour necrosis factor alpha (TNF-alpha), interferon gamma (IFN-gamma) and transforming growth factor beta 1 (TGF-beta 1) mRNA was analysed in colon and small intestinal tissue of both IL-2 deficient (IL-2-/-) mice and normal (wild type) litter mates (IL-2+/+) at different ages by using qualitative, as well as semiquantitative, competitive reverse transcription polymerase chain reaction (RT-PCR). Results were correlated with the phase of progression of the disease, as determined by histology. Results-IL-2-/- mice had expressed low levels of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, and IFN-gamma mRNA in the colon by 1.5weeks of age. In advance of the development of histologically and clinically detectable bowel inflammation, expression of IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IFN-gamma, and IL-10, but not TGF-beta 1, mRNA increased in the colon of IL-2 deficient mice. In contrast, IL-2+/+ mice expressed TGF-beta 1 mRNA in colon tissue at 13 and 23 weeks of age, but not IL-1 alpha, IL-1 beta, IL-6, TNF-alpha, IL-10, or IFN-gamma mRNA. Levels of expression of cytokine mRNA in tissue from the small intestine were comparable in IL-2-/- and IL-2+/+ mice. Conclusions-Bowel inflammation in IL-2 deficient mice is preceded by an increase in IL-1 alpha, IL-1 beta, TNF-alpha, and IFN-gamma mRNA expression in colontissue. Low levels of TGF-beta 1, but high levels of IL-1 alpha, IL-1beta, IL-6, TNF-alpha, IFN-gamma, and IL-10 mRNA expression correlatewith the manifestation of severe colitis, and suggest that T cells and macrophages are involved in bowel inflammation of IL-2 deficient mice.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 11:18:17