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Titolo:
COMPLEMENT-MEDIATED ENHANCEMENT OF HIV-1 INFECTION IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS
Autore:
NIELSEN SD; SORENSEN AMM; SCHONNING K; LUND O; NIELSEN JO; HANSEN JES;
Indirizzi:
HVIDOVRE UNIV HOSP,INFECT DIS LAB DK-2650 HVIDOVRE DENMARK HVIDOVRE UNIV HOSP,INFECT DIS LAB DK-2650 HVIDOVRE DENMARK
Titolo Testata:
Scandinavian journal of infectious diseases
fascicolo: 5, volume: 29, anno: 1997,
pagine: 447 - 452
SICI:
0036-5548(1997)29:5<447:CEOHII>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-IMMUNODEFICIENCY-VIRUS; ANTIBODY-DEPENDENT ENHANCEMENT; ENHANCING ANTIBODIES; TYPE-1 INFECTION; LYMPHOCYTES-T; NEUTRALIZATION; SERUM; EXPRESSION; RECEPTORS; BINDING;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
S.D. Nielsen et al., "COMPLEMENT-MEDIATED ENHANCEMENT OF HIV-1 INFECTION IN PERIPHERAL-BLOOD MONONUCLEAR-CELLS", Scandinavian journal of infectious diseases, 29(5), 1997, pp. 447-452

Abstract

We investigated if complement-mediated enhancement of HIV infection occurs in peripheral blood mononuclear cells (PBMC). In 7 experiments, me evaluated the effect of human complement on HIVIIIB infection in vitro. We measured HIV antigen production on day 4 and found that pre-incubation of HIV with complement led to enhanced production of antigen with a median enhancement of 2.5-fold (range 1.1-6.8). This complement-mediated increase in antigen production was statistically significant(p < 0.02). Complement-mediated enhancement of HIV infection was alsotested in CD4 cells enriched from PBMC, and CD4 cells persistently gave higher levels of infection enhancement than PBMC, Thus, CD4 cells appear to be sufficient for complement-mediated enhancement of HIV infection to occur, In addition, me tested if it mas possible to detect complement-mediated enhancement of primary HIV isolates in PBMC. We tested 3 isolates and found only a minor effect on antigen production (median enhancement 1.2-fold, range 0.6-1.5). Furthermore, addition of HIV-specific antibodies in combination with complement resulted in enhanced antigen production in 2/3 sera tested. However, the combination of complement and antibodies resulted in only a minor increase in enhancement of HIV infection compared to that obtained with complement alone. Finally, me found evidence of complement-mediated enhancement of HIV infection in resting PBMC. In conclusion, me demonstrated that complement-mediated enhancement of HIV infection does occur in vitro in PBMC.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 05:36:39