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Titolo:
PURIFICATION AND CHARACTERIZATION OF AN INTRACELLULAR ESTERASE FROM BREVIBACTERIUM-LINENS ATCC-9174
Autore:
RATTRAY FP; FOX PF;
Indirizzi:
NATL UNIV IRELAND UNIV COLL CORK,DEPT FOOD CHEM CORK IRELAND
Titolo Testata:
International dairy journal
fascicolo: 4, volume: 7, anno: 1997,
pagine: 273 - 278
SICI:
0958-6946(1997)7:4<273:PACOAI>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Keywords:
ESTERASE; BREVIBACTERIUM LINENS; CHEESE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
F.P. Rattray e P.F. Fox, "PURIFICATION AND CHARACTERIZATION OF AN INTRACELLULAR ESTERASE FROM BREVIBACTERIUM-LINENS ATCC-9174", International dairy journal, 7(4), 1997, pp. 273-278

Abstract

An intracellular esterase from Brevibacterium linens ATCC 9174 was purified 44-fold to homogeneity using ammonium sulphate fractionation, anion exchange chromatography, hydrophobic interaction chromatography and anion exchange chromatography. The pH and temperature optima were 7.5 and 35 degrees C, respectively. The molecular mass of the enzyme was found to be 54kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis and 201 kDa by gel filtration, indicating that the native enzyme exists as a tetramer. The pI of the purified esterase was 4.0. The esterase was activated by ethylenediaminetetraacetic acid, but strongly inhibited by N-ethylmaleimide and p-hydroxymercuribenzoate, Cd2+, Zn2+ and Hg2+; activity was increased by dithiothreitol and cysteineat 0.1 mM, but decreased at 1.0 mM. The esterase hydrolysed beta-naphthyl esters of acetic, butyric, caproic, caprylic and capric acids, but not of lauric, myristic, palmitic or oleic acids. K-m and k(cat) values for beta-naphthyl butyrate were 0.1 mM and 0.2 s(-1), respectively, while the corresponding values for beta-naphthyl caproate were 0.03 mM and 0.5 s(-1), respectively. The sequence of the first 19 N-terminal amino acids was y-Thr-Glu-Gly-Ser-Leu-Val-Thr-Phe-Lys-Pro-Arg-Tyr. (C) 1997 Elsevier Science Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:18:44