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Titolo:
IDENTIFICATION OF A 2ND MYOSIN-II IN SCHIZOSACCHAROMYCES-POMBE - MYP2P IS CONDITIONALLY REQUIRED FOR CYTOKINESIS
Autore:
BEZANILLA M; FORSBURG SL; POLLARD TD;
Indirizzi:
SALK INST BIOL STUDIES,STRUCT BIOL LAB,10010 N TORREY PINES RD LA JOLLA CA 92037 SALK INST BIOL STUDIES,STRUCT BIOL LAB LA JOLLA CA 92037 SALK INST BIOL STUDIES,MOL BIOL & VIROL LAB LA JOLLA CA 92037
Titolo Testata:
Molecular biology of the cell
fascicolo: 12, volume: 8, anno: 1997,
pagine: 2693 - 2705
SICI:
1059-1524(1997)8:12<2693:IOA2MI>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
PREDICTING COILED COILS; ALPHA-TUBULIN GENES; CELL-DIVISION CYCLE; FISSION YEAST; MAP KINASE; SEPTUM FORMATION; PROTEIN-KINASE; S-POMBE; ENCODES; MITOSIS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
M. Bezanilla et al., "IDENTIFICATION OF A 2ND MYOSIN-II IN SCHIZOSACCHAROMYCES-POMBE - MYP2P IS CONDITIONALLY REQUIRED FOR CYTOKINESIS", Molecular biology of the cell, 8(12), 1997, pp. 2693-2705

Abstract

As in many eukaryotic cells, fission yeast cytokinesis depends on theassembly of an actin ring. We cloned myp2(+), a myosin-II in Schizosaccharomyces pombe, conditionally required for cytokinesis. myp2(+), the second myosin-II identified in S. pombe, does not completely overlapin function with myo2(+). The catalytic domain of Myp2p is highly homologous to known myosin-IIs, and phylogenetic analysis places Myp2p inthe myosin-II family. The Myp2p sequence contains well-conserved ATP-and actin-binding motifs, as well as two IQ motifs. However, the tailsequence is unusual, since it is predicted to form two long coiled-coils separated by a stretch of sequence containing 19 prolines. Disruption of myp2(+) is not lethal but under nutrient limiting conditions cells lacking myp2(+) function are multiseptated, elongated, and branched, indicative of a defect in cytokinesis. The presence of salt enhances these morphological defects. Additionally, Delta myp2 cells are coldsensitive in high salt, failing to form colonies at 17 degrees C. Thus, myp2(+) is required under conditions of stress, possibly linking extracellular growth conditions to efficient cytokinesis and cell growth. GFP-Myp2p localizes to a ring in the middle of late mitotic cells, consistent with a role in cytokinesis. Additionally, we constructed double mutants of Delta myp2 with temperature-sensitive mutant strains defective in cytokinesis. We observed synthetic lethal interactions between Delta myp2 and three alleles of cdc11ts, as well as more modest synthetic interactions with cdc14ts and cdc16ts, implicating myp2(+) function for efficient cytokinesis under normal conditions.

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Documento generato il 30/03/20 alle ore 12:28:43