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Titolo:
PARTHENOGENETIC ACTIVATION OF PIG EGGS BY EXPOSURE TO PROTEIN-KINASE INHIBITORS
Autore:
PRATHER RS; MAYES MA; MURPHY CN;
Indirizzi:
UNIV MISSOURI,DEPT ANIM SCI COLUMBIA MO 65211
Titolo Testata:
Reproduction, fertility and development
fascicolo: 5, volume: 9, anno: 1997,
pagine: 539 - 544
SICI:
1031-3613(1997)9:5<539:PAOPEB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
XENOPUS EGGS; 6-DIMETHYLAMINOPURINE 6-DMAP; INTRACELLULAR CALCIUM; OOCYTES INVITRO; PHORBOL ESTER; MOUSE OOCYTES; HAMSTER EGGS; FERTILIZATION; STAUROSPORINE; TRANSITION;
Keywords:
PARTHENOGENOTE; H7; EGG ACTIVATION; OOCYTE ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
37
Recensione:
Indirizzi per estratti:
Citazione:
R.S. Prather et al., "PARTHENOGENETIC ACTIVATION OF PIG EGGS BY EXPOSURE TO PROTEIN-KINASE INHIBITORS", Reproduction, fertility and development, 9(5), 1997, pp. 539-544

Abstract

The objective of the present study was to assess the effect of low concentrations of protein kinase inhibitors on activation. Pig eggs wereelectrostimulated or cultured with the following: 10 mu M 1-[5-isoquinolinylsulfonyl]-2-methylpiperazine HCl H7 for 24 h; 100 mu M H7 for 24 h; 10 nM staurosporine for 24 h; or with 20 mu M staurosporine for 20 min followed by Whitten's medium for 24 h. Rates of pronuclear formation in eggs (n = 1240) subjected to these treatments were: untreated,6.2%; electrostimulated, 77.1%; 10 mu M H7, 10.0%; 100 mu M H7, 65%; 10 nM staurosporine, 24.2%; and 20 mu M staurosporine, 67.3% (significance at P less than or equal to 0.05: 10 mu M H7 vs untreated, not significant; 20 mu M staurosporine vs 100 mu M H7, not significant). Percentages of eggs (n = 125) expressing a 22-kDa band after treatment were: untreated, 37.5%; electrostimulated, 100%; 10 mu M H7, 72%; 100 mu M H7, 66.7%; 10 nM staurosporine, 40.0%; and 20 mu M staurosporine, 77.3% (significance at P less than or equal to 0.10: 100 mu M H7, 10 nM staurosporine and 20 mu M staurosporine vs 10 mu M H7, not significant; 100 mu M H7 and 10 nM staurosporine vs untreated, not significant). Transmission electron microscopy of ultrathin sections of treated eggsrevealed that cortical granules were present in over half the untreated eggs, as well as over half of the eggs treated with 100 mu M H7 or 10 nM staurosporine; in contrast, all cortical granules were absent from electrically-activated eggs. The results indicate that long-term exposure of eggs to low concentrations of broad-spectrum protein kinase inhibitors induces some of the events commonly associated with fertilization.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 04:48:19