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Titolo:
CHARACTERIZATION AND MODULATION OF LP(A) IN HUMAN HEPATOMA HEPG2 CELLS
Autore:
VU H; CIANFLONE K; ZHANG ZJ; KALANT D; SNIDERMAN AD;
Indirizzi:
MCGILL UNIV,ROYAL VICTORIA HOSP,MIKE ROSENBLOOM LAB CARDIOVASC RES,687 PINE AVE W,ROOM H7-22 MONTREAL PQ H3A 1A1 CANADA MCGILL UNIV,ROYAL VICTORIA HOSP,MIKE ROSENBLOOM LAB CARDIOVASC RES MONTREAL PQ H3A 1A1 CANADA
Titolo Testata:
Biochimica et biophysica acta, L. Lipids and lipid metabolism
fascicolo: 2, volume: 1349, anno: 1997,
pagine: 97 - 108
SICI:
0005-2760(1997)1349:2<97:CAMOLI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
LOW-DENSITY LIPOPROTEINS; FULL-LENGTH CDNA; APO-B SECRETION; HUMAN APOLIPOPROTEIN(A); AMINO-ACIDS; CHOLESTERYL ESTER; HUMAN-PLASMA; HUMAN-LIVER; GENE; PLASMINOGEN;
Keywords:
LIPOPROTEIN LP(A); APOLIPOPROTEIN B100; HEPATOCYTE; LIPID METABOLISM; AMINO ACID;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
53
Recensione:
Indirizzi per estratti:
Citazione:
H. Vu et al., "CHARACTERIZATION AND MODULATION OF LP(A) IN HUMAN HEPATOMA HEPG2 CELLS", Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1349(2), 1997, pp. 97-108

Abstract

HepG2 cells have been widely used to study factors which affect the secretion of apoB100 lipoprotein particles. The objectives of this study were to determine if Lp(a) particles were present in conditioned medium from HepG2 cells and if so, was this accumulation affected by factors which alter apoB100 lipoprotein metabolism. The data demonstrate that Lp(a) accumulated in the medium in a time dependent manner over a 48 h incubation period. Ultracentrifugation fractionation and Western blot analysis demonstrated that lipoprotein particles containing apo(a) in complex with apoB100 were present at a density consistent with human plasma Lp(a). Incubation of the HepG2 cells with LDL or VLDL caused increases in Lp(a) accumulation in the medium (+33% +/- 14%, P NS and 56% +/- 21%, P < 0.05, respectively). In contrast, apo(a) mRNA decreased (-17% +/- 3%, P < 0.01 for both LDL and VLDL incubation). Increasing concentrations of amino acids in the medium resulted in progressively less Lp(a) and apoB100 in the medium, the effect being greater on apoB100. ApoB100 mRNA levels decreased with incubation of HepG2 cells with amino acids (-22% +/- 10%, P < 0.06) whereas apo(a) mRNA levels increased significantly (+47% +/- 14%, P < 0.005). Taken together, our data show that HepG2 cells express mRNA for apo(a), and accumulate Lp(a) in the medium. The close correlation of medium Lp(a) levels with medium apoB100 levels, and not with apo(a) mRNA levels, suggests that medium Lp(a) accumulation may be a function of lipoprotein synthesis andsecretion and is consistent with extracellular assembly of Lp(a) lipoprotein particles. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/04/20 alle ore 07:29:26