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Titolo:
IMMUNOGENICITY OF CHIMERIC MULTIPLE ANTIGEN PEPTIDES BASED ON PLASMODIUM-FALCIPARUM ANTIGENS - IMPACT OF EPITOPE ORIENTATION
Autore:
AHLBORG N; NARDIN EH; PERLMANN P; BERZINS K; ANDERSSON R;
Indirizzi:
UNIV STOCKHOLM,DEPT IMMUNOL S-10691 STOCKHOLM SWEDEN NYU,SCH MED,DEPT MED & MOL PARASITOL NEW YORK NY 00000 KAROLINSKA INST,MICROBIOL & TUMORBIOL CTR STOCKHOLM SWEDEN
Titolo Testata:
Vaccine
fascicolo: 1, volume: 16, anno: 1998,
pagine: 38 - 44
SICI:
0264-410X(1998)16:1<38:IOCMAP>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MONOCLONAL-ANTIBODY 33G2; INHIBIT PARASITE GROWTH; HUMAN MALARIA PARASITES; B-CELL EPITOPES; CIRCUMSPOROZOITE PROTEIN; T-CELL; VACCINE; SEQUENCES; SPOROZOITES; MEMBRANE;
Keywords:
PLASMODIUM FALCIPARUM; T CELL-B CELL COLLABORATION; MULTIPLE ANTIGEN PEPTIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
33
Recensione:
Indirizzi per estratti:
Citazione:
N. Ahlborg et al., "IMMUNOGENICITY OF CHIMERIC MULTIPLE ANTIGEN PEPTIDES BASED ON PLASMODIUM-FALCIPARUM ANTIGENS - IMPACT OF EPITOPE ORIENTATION", Vaccine, 16(1), 1998, pp. 38-44

Abstract

Assembly of B and T epitopes in multiple antigen peptides (MAP) can bypass genetically predisposed unresponsiveness to B epitopes. Althoughthe underlying mechanisms are unknown, B-cell responses to such diepitope MAP are influenced by intramolecular epitope orientation. In thisstudy, MAP constructs were synthesized, encompassing two epitopes derived from the Plasmodium falciparum antigens circumsporozoite protein (CS) and Pf332. In addition to B epitopes, the sequences comprised T epitopes restricted to mouse H-2(b) (CS) or to H-2(d) and H-2(k) (Pf332) haplotypes. Congenic H-2(b), H-2(d) and H-2(k) Balb mice were immunized with MAP in which the two epitopes were arranged either tandemly or in parallel. Tandemly arranged (B-T)(4) MAP in which the relevant T epitope was positioned adjacent to the lysine core [(Pf332-CS)(4)-corefor H-2(b) mice and (CS-Pf332)(4)-core for H-2(d) and H-2(k) mice], elicited the most potent antibody responses in terms of reactivity to both epitopes. Additionally, the (B-T)(4) constructs were generally most efficient in recalling proliferative T-cell responses in vitro, irrespective of the MAP used for in vivo priming. As high antibody titers were generated to both epitopes, the position of B epitopes in the constructs does not appear to be critical for an efficient B-cell response. Rather, the association of strong B-and T-cell responses to the (B-T)(4) MAP constructs suggests that the intramolecular position of the relevant T epitope determines the magnitude of specific antibody production. (C) 1997 Elsevier Science Ltd.

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Documento generato il 29/03/20 alle ore 09:16:56