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Titolo:
IN-VITRO TOXICITY OF GLUTEN PEPTIDES IN CELIAC-DISEASE ASSESSED BY ORGAN-CULTURE
Autore:
SHIDRAWI RG; DAY P; PRZEMIOSLO R; ELLIS HJ; NELUFER JM; CICLITIRA PJ;
Indirizzi:
UNITED MED & DENT SCH,ST THOMAS HOSP,GASTROENTEROL UNIT LONDON SE1 7EH ENGLAND UNITED MED & DENT SCH,ST THOMAS HOSP,GASTROENTEROL UNIT LONDON SE1 7EH ENGLAND
Titolo Testata:
Scandinavian journal of gastroenterology
fascicolo: 8, volume: 30, anno: 1995,
pagine: 758 - 763
SICI:
0036-5521(1995)30:8<758:ITOGPI>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELIAC-DISEASE; GLIADIN PEPTIDES; A-GLIADIN; SYNTHETIC DODECAPEPTIDE; ALPHA-GLIADIN; BETA-GLIADIN; INVITRO; SEQUENCE; ADENOVIRUS-12; FRACTIONS;
Keywords:
CELIAC DISEASE; GLIADIN PEPTIDES; ORGAN CULTURE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
R.G. Shidrawi et al., "IN-VITRO TOXICITY OF GLUTEN PEPTIDES IN CELIAC-DISEASE ASSESSED BY ORGAN-CULTURE", Scandinavian journal of gastroenterology, 30(8), 1995, pp. 758-763

Abstract

Background: We have used organ culture to investigate the in vitro toxicity of three oligopeptides corresponding to amino acids 31-49 (peptide A), 202-220 (peptide B), and 3-21 (peptide C) of A-gliadin, Frazer's fraction III (FFIII), and ovalbumin. Methods: Eight to 14 jejunal biopsy specimens were obtained from each of 8 treated and 7 untreated coeliac patients and 5 normal controls and cultured for 18 h in organ culture with test peptide (1 mg/ml) or medium alone. Mean enterocyte cell heights (ECH) were compared with paired values for specimens grown in medium alone. Results: A significant reduction in the mean of the ECH values for each of the patient groups was observed with peptide A and FFIII in both treated (p = 0.01 and 0.02, respectively) and untreated (p = 0.03 and 0.01) coeliac patients when compared with tissue incubated with medium alone. No significant changes in the mean ECH value were noted in any of the patient groups in tissue incubated with peptide B, peptide C, or ovalbumin as compared with those with medium alone. Conclusions: These results suggest that peptide A is toxic in vitro to the jejunal mucosa of both treated and untreated coeliac patients, correlating with recent findings that this peptide exacerbates coeliacdisease in vivo.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 22:20:25