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Titolo:
TISSUE-SPECIFIC PROMOTER USAGE IN THE D-1A DOPAMINE-RECEPTOR GENE IN BRAIN AND KIDNEY
Autore:
LEE SH; WANG W; YAJIMA S; JOSE PA; MOURADIAN MM;
Indirizzi:
NINCDS,ETB,GENET PHARMACOL UNIT,NIH,BLDG 10,ROOM 5C116,10 CTR DR,MSC 1406 BETHESDA MD 20892 NINCDS,ETB,GENET PHARMACOL UNIT,NIH BETHESDA MD 20892 GEORGETOWN UNIV,SCH MED,DEPT PEDIAT WASHINGTON DC 20007
Titolo Testata:
DNA and cell biology
fascicolo: 11, volume: 16, anno: 1997,
pagine: 1267 - 1275
SICI:
1044-5498(1997)16:11<1267:TPUITD>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROXIMAL CONVOLUTED TUBULE; K+-ATPASE ACTIVITY; MOLECULAR-CLONING; TRANSCRIPTIONAL ACTIVATORS; ADENYLATE-CYCLASE; EXPRESSION; D1; RAT; REGION; DNA;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
S.H. Lee et al., "TISSUE-SPECIFIC PROMOTER USAGE IN THE D-1A DOPAMINE-RECEPTOR GENE IN BRAIN AND KIDNEY", DNA and cell biology, 16(11), 1997, pp. 1267-1275

Abstract

The D-1A dopamine receptor gene consists of a short, noncoding exon 1separated from a longer coding exon 2 by a small intron, Recently, wefound that in addition to its original TATA-less promoter located upstream of exon 1, the human D-1A dopamine receptor gene is transcribed in neural cells from a second strong promoter located In its intron, In the present study, we addressed the possibility that these two promoters are used for the tissue-specific regulation of the D-1A gene in neuronal and renal cells, Reverse transcription polymerase chain reaction revealed that D-1A transcripts in the kidneys of humans and rats lack exon 1, Transient transfection analysis of these two promoters in D-1A-expressing cells indicated that the upstream promoter has no detectable activity in the opossum kidney (OK) cell line, in contrast to its strong activity in two neuronal cell lines, SK-N-MC and NS20Y, On the other hand, the D-1A intron promoter showed transcriptional activityboth in OK cells and in neuronal cells, The activator sequence AR1, which enhances transcription from the upstream promoter in SK-N-MC and NS20Y cells, could not activate this promoter in OK cells, In addition, no protein binding to AR1 could be detected by gel mobility shift assay using nuclear extracts from either OK cells or from rat kidney tissue, These findings indicate that the differential expression of shortand long D-1A transcripts is due, at least in part, to the tissue-specific expression of the activator protein binding to AR1 driving transcription from the upstream promoter, Absence of this activator proteinaccounts for the nonfunctional D-1A upstream promoter in the kidney.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:06:24