Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
LONG-TERM BLOCKADE OF CHOLECYSTOKININ (CCK) - EFFECTS OF L-364,718 (ACCK RECEPTOR ANTAGONIST) ON PANCREATIC-ENZYME STORAGE AND SECRETION
Autore:
RODRIGUEZ AI; MANSO MA; GARCIAMONTERO AC; ORFAO A; DEDIOS I;
Indirizzi:
UNIV SALAMANCA,FAC BIOL,DEPT FISIOL & FARMACOL,EDIFICIO DEPT,CAMPUS MIGUEL UNAMUNO SALAMANCA 37007 SPAIN UNIV SALAMANCA,FAC BIOL,DEPT FISIOL & FARMACOL,EDIFICIO DEPT SALAMANCA 37007 SPAIN UNIV SALAMANCA,FLOW CYTOMETRY SERV E-37008 SALAMANCA SPAIN
Titolo Testata:
Pancreas
fascicolo: 3, volume: 15, anno: 1997,
pagine: 314 - 322
SICI:
0885-3177(1997)15:3<314:LBOC(->2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
ISOLATED ZYMOGEN GRANULES; FLOW-CYTOMETRY; RAT PANCREAS; PLASMA CHOLECYSTOKININ; GROWTH; L-364,718; CERULEIN; L-364718; INVIVO; POTENT;
Keywords:
CHOLECYSTOKININ (CCK) RECEPTOR ANTAGONIST; CCK; EXOCRINE PANCREAS; ZYMOGEN GRANULES; RAT;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
A.I. Rodriguez et al., "LONG-TERM BLOCKADE OF CHOLECYSTOKININ (CCK) - EFFECTS OF L-364,718 (ACCK RECEPTOR ANTAGONIST) ON PANCREATIC-ENZYME STORAGE AND SECRETION", Pancreas, 15(3), 1997, pp. 314-322

Abstract

To determine the effect of long-term blockade of cholecystokinin (CCK) on both pancreatic storage and secretion processes, L 364,718 (a CCKreceptor antagonist) was administered to rats at 0.1 mg/kg/day for 3,7, and 15 days. Zymogen granules were analyzed by flow cytometry to determine their light scattering properties-forward scatter and side scatter-as well as their amylase content measured by a specific antiserum. The mean number of zymogen granules per cell was counted on pancreatic sections using electron microscopy. DNA content, pancreatic weight, and enzyme secretion were also studied under both basal conditions and CCK infusion at a dose of 1.25 mu g/kg/h, which is able to displacethe CCK receptor antagonist. Two subpopulations of zymogen granules (Z(1) and Z(2)) were identified on the basis of their light scattering parameters, in both control and L 364,718-treated rats. L 364,718 administered for 3, 7, and 15 days induced a significant reduction in the amylase content of individual zymogen granules, for both Z(1) and Z(2)zymogen granule subsets. In contrast, the number of zymogen granules per cell increased from day +3 of treatment onward, the highest valuesbeing detected at day +7. Hyperplasia was observed only at day +15. Basal enzyme secretion decreased significantly in rats treated with L 364,718 for 3 and 7 days but recovered to control values after 15 days of treatment. No significant differences in CCK-stimulated amylase secretion were observed between control and L 364,718-treated rats. At day +15 of L 364,718 treatment a significant increase in enzyme secretion was observed with respect to shorter treatment periods; this was associated with a significant increase in both the number of cells and the number of zymogen granules per cell. Our results indicate that chronic administration of L 364,718 induces a biphasic effect on pancreaticfunction. interestingly, although enzyme secretion reached recovery after long-term treatment (15 days), the storage process is altered since the mean enzyme content in each individual zymogen granule remains significantly reduced.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:41:59