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Titolo:
EFFICACY OF DIFFERENT METHODS FOR DETECTION OF LOW CRYPTOSPORIDIUM-PARVUM OOCYST NUMBERS OR ANTIGEN CONCENTRATIONS IN STOOL SPECIMENS
Autore:
IGNATIUS R; EISENBLATTER M; REGNATH T; MANSMANN U; FUTH U; HAHN H; WAGNER J;
Indirizzi:
FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,INST MED MIKROBIOL & INFEKT IMMUNOL D-12203 BERLIN GERMANY FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,INST MED STAT & INFORMAT ARBEITUNG D-12203 BERLIN GERMANY AUGUSTE VIKTORIA KRANKENHAUS,ZENT LAB D-12157 BERLIN GERMANY
Titolo Testata:
European journal of clinical microbiology & infectious diseases
fascicolo: 10, volume: 16, anno: 1997,
pagine: 732 - 736
SICI:
0934-9723(1997)16:10<732:EODMFD>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTESTINAL PARASITES; DIAGNOSTIC METHODS; FECAL SPECIMENS; IDENTIFICATION; IMMUNOASSAY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
R. Ignatius et al., "EFFICACY OF DIFFERENT METHODS FOR DETECTION OF LOW CRYPTOSPORIDIUM-PARVUM OOCYST NUMBERS OR ANTIGEN CONCENTRATIONS IN STOOL SPECIMENS", European journal of clinical microbiology & infectious diseases, 16(10), 1997, pp. 732-736

Abstract

The detection of Cryptosporidium parvum oocysts in stool specimens byacid-fast (AF) stains or immunofluorescence assays (IFA) requires thepresence of large numbers of oocysts. To determine whether new commercially available enzyme immunoassays (EIAs) are more sensitive alternatives, three EIAs, a direct IFA, and the modified cold Kinyoun AF stain were compared, particularly with respect to detection of low oocyst numbers or antigen concentrations. Thirty-one negative and 31 calf stool-enriched human stool specimens were tested. One EIA method detectedonly nine positive specimens, demonstrating a sensitivity significantly less (p < 0.0001) than that of the IFA, the AF stain, and the othertwo EIAs. No differences could be found with respect to specificity. In addition, serial dilutions of 28 patients' stool samples containingcryptosporidian oocysts were prepared and examined using two EIAs, [FA, and the AF stain. One EIA yielded significantly inferior results (p< 0.0001), whereas the other one and the two microscopic methods did not differ significantly in either part of the study, The results indicate that the new EIAs do not exhibit higher sensitivities for detection of Cryptosporidium parvum than the two routinely used microscopic methods. Thus, for most-laboratories, the IFA or AF stain may still represent the preferred method for the diagnosis of cryptosporidiosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/21 alle ore 07:50:43