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Titolo:
EPIDERMAL GROWTH-FACTOR INDUCES INCREASED MUCOSAL THICKNESS OF THE SMALL-INTESTINE IN MOUSE
Autore:
JANSEN C; IHSE I; AXELSON J;
Indirizzi:
UNIV LUND HOSP,DEPT SURG S-22185 LUND SWEDEN UNIV LUND HOSP,DEPT SURG S-22185 LUND SWEDEN
Titolo Testata:
European surgical research
fascicolo: 6, volume: 29, anno: 1997,
pagine: 447 - 454
SICI:
0014-312X(1997)29:6<447:EGIIMT>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PANCREATIC SECRETIONS; RAT; CELLS; PROLIFERATION; RECEPTORS; RELEASE; CANCER; BILE; EGF;
Keywords:
EPIDERMAL GROWTH FACTOR; STOMACH; SMALL INTESTINE; COLON; MUCOSAL THICKNESS; TRITIATED THYMIDINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
C. Jansen et al., "EPIDERMAL GROWTH-FACTOR INDUCES INCREASED MUCOSAL THICKNESS OF THE SMALL-INTESTINE IN MOUSE", European surgical research, 29(6), 1997, pp. 447-454

Abstract

Epidermal growth factor (EGF) is known to exert a mitogenic effect indifferent tissues, including the digestive tract. The aim of the present study was to evaluate whether long-term infusion of EGF causes trophic effects in the gastrointestinal tract of female mice. The animalswere infused subcutaneously in the neck with human recombinant EGF ina dose of 10 mu g/kg/h (1.6 nmol/kg/h) using an osmotic minipump for 1, 3 and 7 days, respectively. Tritiated thymidine was continuously infused intraperitoneally during the same period, except in the 7-day group, where it was infused during the last 3 days. The mucosal thickness was measured microscopically. As a measurement of DNA synthesis, theamount of thymidine retained in the mucosa was registered using a scintillation counter. After 1 day of EGF infusion, the mucosal thicknesswas increased in the antrum and, after 3 days, in the fundus. In the proximal duodenum, an increased depth of the crypts was seen after 1 day, followed by increased villi height after 3 and 7 days; in the distal duodenum, EGF evoked increased villi height after 3 and 7 days. Theheight of villi was increased after 7 days in the jejunum and ileum in the ECF-treated animals. The tritium incorporation was increased in the fundus of the stomach and the proximal duodenum in the EGF-treatedanimals after 3 days, whereas no significant increase in tritiated thymidine incorporation could be detected in the EGF-treated animals after 1 and 7 days compared to the controls. In conclusion, continuous infusion of EGF evoked increased mucosal thickness in the small intestine, while the trophic effects were only of a short duration in the stomach and absent in the colon.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 14:47:42