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Titolo:
OBSERVATIONS ON INITIAL CELL LOSS AFTER INTRAPORTAL HEPATOCYTE TRANSPLANTATION OF 5'-BROMO-DEOXY-URIDINE-LABELED HEPATOCYTES
Autore:
KOCKEN JM; BOUWMAN E; RINKES IHMB; SINAASAPPEL M; TERPSTRA OT;
Indirizzi:
LEIDEN UNIV,MED CTR,DEPT SURG,P1-Q,POB 9600 NL-2300 RC LEIDEN NETHERLANDS ERASMUS UNIV ROTTERDAM,DEPT PEDIAT,DIV GASTROENTEROL ROTTERDAM NETHERLANDS UNIV ROTTERDAM HOSP,SOPHIA CHILDRENS HOSP ROTTERDAM NETHERLANDS UNIV UTRECHT HOSP,DEPT SURG UTRECHT NETHERLANDS
Titolo Testata:
European surgical research
fascicolo: 6, volume: 29, anno: 1997,
pagine: 411 - 420
SICI:
0014-312X(1997)29:6<411:OOICLA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NORMAL RAT-LIVER; EXTRACELLULAR-MATRIX; GENE-THERAPY; DEFICIENT RABBITS; REPOPULATION; CYCLOSPORINE; MACROPHAGES; SPLEEN; REGENERATION; ENGRAFTMENT;
Keywords:
HEPATOCYTE TRANSPLANTATION, INTRAPORTAL; RATS, SYNGENEIC; 5'-BROMO-DEOXY-URIDINE; QUANTIFICATION; DISTRIBUTION; INITIAL CELL LOSS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Kocken et al., "OBSERVATIONS ON INITIAL CELL LOSS AFTER INTRAPORTAL HEPATOCYTE TRANSPLANTATION OF 5'-BROMO-DEOXY-URIDINE-LABELED HEPATOCYTES", European surgical research, 29(6), 1997, pp. 411-420

Abstract

In the present study, attempts are made to improve posttransplant survival of intraportally transplanted hepatocytes in a syngeneic hepatocyte transplantation (HTX) model. Engrafted hepatocytes were detected and quantified after pretransplant labeling with 5'-bromo-deoxy-uridine. In order to enhance the survival percentage, three mechanisms that possibly influence cell survival were manipulated: (1) administration of Matrigel(TM) (extracellular-matrix components, Matrigel Basement Membrane Complex, Micronic BV, Lelystad, The Netherlands) in order to improve the environmental conditions of the transplanted hepatocytes; (2)immunosuppression of the recipients by 5 Gy total body irradiation and cyclosporin-A administration (25 mg/kg, 3 x weekly), and (3) Kupffercell depletion by injection of dichloromethylene-diphosphonate-filledliposomes. The results were analyzed in relation to HTX without treatment. Cell survival was similar to 14% (10 days after HTX), and the transplanted hepatocytes were distributed equally throughout the variousrecipient liver lobes. However, no increase was attained by Matrigel coadministration, immunosuppression, or Kupffer cell depletion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 00:14:36