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Titolo:
TRANSITION OF SERUM VITELLOGENIN CYCLE IN SAKHALIN TAIMEN (HUCHO-PERRYI)
Autore:
HIRAMATSU N; SHIMIZU M; FUKADA H; KITAMURA M; URA K; FUDA H; HARA A;
Indirizzi:
HOKKAIDO UNIV,FAC FISHERIES,NANAE FISH CULTURE EXPT STN NANAE HOKKAIDO 04111 JAPAN HOKKAIDO UNIV,FAC FISHERIES,NANAE FISH CULTURE EXPT STN NANAE HOKKAIDO 04111 JAPAN HOKKAIDO UNIV,FAC FISHERIES,DEPT BIOL & AQUACULTURE HAKODATE HOKKAIDO041 JAPAN HOKKAIDO UNIV,SCH MED,LAB MED SAPPORO HOKKAIDO 060 JAPAN
Titolo Testata:
Comparative biochemistry and physiology. C. Comparative pharmacologyand toxicology
fascicolo: 2, volume: 118, anno: 1997,
pagine: 149 - 157
SICI:
1367-8280(1997)118:2<149:TOSVCI>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
CATFISH ICTALURUS-PUNCTATUS; BASS MORONE-SAXATILIS; EGG-YOLK PROTEINS; RAINBOW-TROUT; SALMO-GAIRDNERI; OVARIAN FOLLICLES; QUANTIFICATION; IMMUNOASSAY; ESTRADIOL; INDUCTION;
Keywords:
AVIDIN; BIOTIN; ELISA; LIPOVITELLIN; PRIMARY REACTION; SAKHALIN TAIMEN; VITELLOGENIN;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
N. Hiramatsu et al., "TRANSITION OF SERUM VITELLOGENIN CYCLE IN SAKHALIN TAIMEN (HUCHO-PERRYI)", Comparative biochemistry and physiology. C. Comparative pharmacologyand toxicology, 118(2), 1997, pp. 149-157

Abstract

A specific and sensitive enzyme linked immunosorbent assay (ELISA) and a single radial immunodiffusion (SRID) were developed for measurement of serum vitellogenin (Vg) levels in Sakhalin taimen (Hucho perryi). Regarding specificity for serum Vg, an antiserum raised against lipovitellin of taimen (a-Lv) was adequate for both assays. ELISA and SRID could detect Vg in serum at concentrations as low as 10 ng/ml and 25 mu g/ml, respectively. In estrogen administration experiments, the level of serum Vg began clearly increasing within 12 to 24 hr after injection of immature females with estradiol-17 beta (E-2) The appearance and levels of Vg in males treated with E-2 were delayed and smaller, respectively, than for females. Vg levels varied throughout natural vitellogenesis from 0-4 mu g/ml (3 years old) to approximately 30 mg/ml (5-6 years old). We observed an early transitory peak of serum Vg levels (primary reaction) at the time of early vitellogenesis and chronic high Vg levels (for 6-7 months) in winter period before ovulation. Changes of serum E-2 levels were correlated with Vg levels. However, E-2 levels decreased a month earlier than Vg levels near ovulation. It appears that the duration of vitellogenesis in taimen is considerably longerthan that in other salmonids, lasting more than 2 years. (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 15:49:11