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Titolo:
STRUCTURE OF CYTOCHROME C' FROM RHODOBACTER-CAPSULATUS STRAIN ST-LOUIS - AN UNUSUAL MOLECULAR ASSOCIATION INDUCED BY BRIDGING ZN IONS
Autore:
TAHIROV TH; MISAKI S; MEYER TE; CUSANOVICH MA; HIGUCHI Y; YASUOKA N;
Indirizzi:
OSAKA UNIV,INST PROT RES,YAMADAOKA 3-2 SUITA OSAKA 565 JAPAN AZERBAIJAN ACAD SCI,INST INORGAN & PHYS CHEM BAKU 370143 AZERBAIJAN UNIV ARIZONA,DEPT BIOCHEM TUCSON AZ 85721
Titolo Testata:
Acta crystallographica. Section D, Biological crystallography
, volume: 53, anno: 1997,
parte:, 6
pagine: 658 - 664
SICI:
0907-4449(1997)53:<658:SOCCFR>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
RHODOSPIRILLUM-MOLISCHIANUM; FERRICYTOCHROME C'; X-RAY; HEME PROTEIN; RESOLUTION; CRYSTALLIZATION; DISSOCIATION; REPLACEMENT; REFINEMENT; ISOCYANIDE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
T.H. Tahirov et al., "STRUCTURE OF CYTOCHROME C' FROM RHODOBACTER-CAPSULATUS STRAIN ST-LOUIS - AN UNUSUAL MOLECULAR ASSOCIATION INDUCED BY BRIDGING ZN IONS", Acta crystallographica. Section D, Biological crystallography, 53, 1997, pp. 658-664

Abstract

Rhodobacter capsulatus strain St Louis cytochrome c' (RCCP-SL) has been crystallized and the structure solved by molecular replacement. It was refined at 2.1 Angstrom resolution to an R value of 18.4%, and compared with Rhodobacter capsulatus strain M110 cytochrome c' (RCCP-M110). Although these two proteins are very similar in sequence and structure, the intermolecular interaction is largely different. In RCCP-M110, the molecules dimerize through interaction of helix B to form an antiparallel arrangement. When crystallized in the presence of Zn ions, molecules of RCCP-SL were found to be arranged as linear polymers connected by the bridging Zn ions. The changes in conformation of the side chains induced by binding of the Zn ions, by the substitution of Glu90for Asp90, and by the different arrangement of the molecules, are discussed in detail.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 16:28:06