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Titolo:
PLACEMENT OF THE ALPHA-SARCIN LOOP WITHIN THE 50S SUBUNIT - EVIDENCE DERIVED USING A PHOTOLABILE OLIGODEOXYNUCLEOTIDE PROBE
Autore:
MURALIKRISHNA P; ALEXANDER RW; COOPERMAN BS;
Indirizzi:
UNIV PENN,DEPT CHEM PITTSBURGH PA 19104 UNIV PENN,DEPT CHEM PITTSBURGH PA 19104
Titolo Testata:
Nucleic acids research
fascicolo: 22, volume: 25, anno: 1997,
pagine: 4562 - 4569
SICI:
0305-1048(1997)25:22<4562:POTALW>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
23S RIBOSOMAL-RNA; ELONGATION-FACTOR-G; ESCHERICHIA-COLI RIBOSOME; OLIGODEOXYRIBONUCLEOTIDE PROBE; EUKARYOTIC RIBOSOMES; SPATIAL ARRANGEMENT; MONOCLONAL-ANTIBODY; RICIN LOOP; FACTOR TU; BINDING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
P. Muralikrishna et al., "PLACEMENT OF THE ALPHA-SARCIN LOOP WITHIN THE 50S SUBUNIT - EVIDENCE DERIVED USING A PHOTOLABILE OLIGODEOXYNUCLEOTIDE PROBE", Nucleic acids research, 25(22), 1997, pp. 4562-4569

Abstract

We report the synthesis of a radioactive, photolabile oligodeoxyribonucleotide probe and its exploitation in identifying 50S ribosomal subunit components neighboring the alpha-sarcin loop, The probe is complementary to 23S rRNA nt 2653-2674, Photolysis of the complex formed between the probe and 50S subunits leads to site-specific probe photoincorporation into proteins L2, the most highly labeled protein, L1, L15, L16 and L27, labeled to intermediate extents, and L5, L9, L17 and L24, each labeled to a minor extent, Portions of each of these proteins thus lie within 23 Angstrom of nt U2653, These results lead us to conclude that the alpha-sarcin loop is located at the base of the L1 projection within the 50S subunit. Such placement, near the peptidyl transferase center, provides a rationale for the extreme sensitivity of ribosomal function to cleavage of the alpha-sarcin loop.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:06:08