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Titolo:
ASSAY FOR QUANTITATION OF CLOZAPINE AND ITS METABOLITE N-DESMETHYLCLOZAPINE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION
Autore:
EDNO L; COMBOURIEU I; CAZENAVE M; TIGNOL J;
Indirizzi:
CTR HOSP CHARLES PERRENS,BIOL LAB,121 RUE BECHADE F-33076 BORDEAUX FRANCE UNIV BORDEAUX 2,LAB PSYCHIAT F-33076 BORDEAUX FRANCE
Titolo Testata:
Journal of pharmaceutical and biomedical analysis
fascicolo: 2, volume: 16, anno: 1997,
pagine: 311 - 318
SICI:
0731-7085(1997)16:2<311:AFQOCA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-CHROMATOGRAPHY; HUMAN-SERUM;
Keywords:
CLOZAPINE; N-DESMETHYLCLOZAPINE; HPLC; DRUG MONITORING; HUMAN PLASMA;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
19
Recensione:
Indirizzi per estratti:
Citazione:
L. Edno et al., "ASSAY FOR QUANTITATION OF CLOZAPINE AND ITS METABOLITE N-DESMETHYLCLOZAPINE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION", Journal of pharmaceutical and biomedical analysis, 16(2), 1997, pp. 311-318

Abstract

A high-performance liquid chromatographic method with UV detection has been developed for the analysis of clozapine and its active N-desmethylated metabolite (N-desmethylclozapine = DMC) in human plasma. A liquid/liquid procedure was used to extract clozapine and DMC from human plasma. The analysis was performed on a Cs Nucleosil column and the mobile phase comprised acetonitrile-water-Pic B5 diethylamine (63:37:25:0.04, v/v/v/v). The detection wavelength was 245 nm. The intra-assay and inter-assay precision was satisfactory within the concentration range 10-900 ng ml(-1). The lower detection limit for clozapine and for DMC was 5 ng ml(-1). The recovery and reproducibility values of this method were better or similar to those found by other authors. This method, which is simple, selective and avoids an evaporation step, can be used routinely for therapeutic drug monitoring. (C) 1997 Elsevier Science B.V.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 19:18:05