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Titolo:
EFFECT OF EMBRYONIC DONOR AGE AND DISSECTION ON THE DARPP-32 CONTENT OF CELL-SUSPENSIONS USED FOR INTRASTRIATAL TRANSPLANTATION
Autore:
WATTS C; DUNNETT SB; ROSSER AE;
Indirizzi:
UNIV CAMBRIDGE,ACAD DEPT NEUROSURG CAMBRIDGE CB2 2PY ENGLAND UNIV CAMBRIDGE,MRC,CAMBRIDGE CTR BRAIN REPAIR CAMBRIDGE CB2 2PY ENGLAND
Titolo Testata:
Experimental neurology
fascicolo: 1, volume: 148, anno: 1997,
pagine: 271 - 280
SICI:
0014-4886(1997)148:1<271:EOEDAA>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
LATERAL GANGLIONIC EMINENCE; HUNTINGTONS-DISEASE; STRIATAL GRAFTS; CEREBRAL-CORTEX; NEURAL TRANSPLANTATION; MIGRATION PATTERNS; CLINICAL-TRIALS; IN-VITRO; NEURONS; RAT;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
59
Recensione:
Indirizzi per estratti:
Citazione:
C. Watts et al., "EFFECT OF EMBRYONIC DONOR AGE AND DISSECTION ON THE DARPP-32 CONTENT OF CELL-SUSPENSIONS USED FOR INTRASTRIATAL TRANSPLANTATION", Experimental neurology, 148(1), 1997, pp. 271-280

Abstract

The aim of this study was to determine in vitro the DARPP-32 content of donor cells used for striatal transplantation in vivo. The effect of selective embryonic dissection of the lateral ganglionic eminence (LGE) was compared with the standard dissection of the whole ganglionic eminence (WGE) at each of three embryonic ages (14, 15, and 16 days ofgestation) in the rat. The resultant cell suspensions were cultured for up to 7 days and incubated with antibodies against DARPP-32, a marker of striatal medium spiny neurons; beta-tubulin III, a neuronal marker; GFAP, a marker of reactive astrocytes; and Gal-C, a marker of oligodendrocytes. LGE dissection gave rise to more DARPP-32 neurons compared to WGE; but this relationship was only observed in the younger embryos. When older (16 days gestation) embryos are used there is no difference in the yield of DARPP-32 cells obtained from LGE and WGE. LGE dissections were also observed to contain fewer glial cells. There was no beneficial effect of LGE over WGE on survival of striatal neurons invitro. These results have important implications for the selection and dissection of fetal donor material used in clinical trials of intrastriatal transplantation as a potential treatment for Huntington's disease. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 07:21:00