Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IN-VITRO CELL DENSITY-DEPENDENT CLONAL GROWTH OF EGF-RESPONSIVE MURINE NEURAL PROGENITOR CELLS UNDER SERUM-FREE CONDITIONS
Autore:
HULSPAS R; TIARKS C; REILLY J; HSIEH CC; RECHT L; QUESENBERRY PJ;
Indirizzi:
UNIV MASSACHUSETTS,MED CTR,DEPT CELL BIOL WORCESTER MA 01605 UNIV MASSACHUSETTS,MED CTR,DEPT MED WORCESTER MA 01605 UNIV MASSACHUSETTS,MED CTR,DEPT NEUROL WORCESTER MA 01605 UNIV MASSACHUSETTS,CTR CANC,DEPT CELL BIOL WORCESTER MA 01605 UNIV MASSACHUSETTS,CTR CANC,DEPT MED WORCESTER MA 01605 UNIV MASSACHUSETTS,CTR CANC,DEPT NEUROL WORCESTER MA 01605
Titolo Testata:
Experimental neurology
fascicolo: 1, volume: 148, anno: 1997,
pagine: 147 - 156
SICI:
0014-4886(1997)148:1<147:ICDCGO>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT CEREBRAL-CORTEX; COMMON PRECURSOR; STEM-CELLS; NEURONS; ASTROCYTES; DIFFERENTIATION; GENERATION; SURVIVAL; LINEAGES; EXPRESS;
Keywords:
NEURAL PRECURSOR; CLONAL ASSAY; NESTIN; GFAP; NEUROFILAMENT; STEM CELLS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
32
Recensione:
Indirizzi per estratti:
Citazione:
R. Hulspas et al., "IN-VITRO CELL DENSITY-DEPENDENT CLONAL GROWTH OF EGF-RESPONSIVE MURINE NEURAL PROGENITOR CELLS UNDER SERUM-FREE CONDITIONS", Experimental neurology, 148(1), 1997, pp. 147-156

Abstract

Neural progenitor cell populations responsive to epidermal growth factor (EGF) have been shown to have proliferative potential and give rise to neurons, astrocytes, and oligodendrocytes. We have characterized EGF-responsive neural progenitor cells that give rise to bilineage neuronal/glial colonies (colony-forming unit neuron-glia; CFU-NeGl) and unilineage neuronal colonies (CFU-Ne). Clonality was confirmed utilizing mixtures of brain cells from Balb/c and ROSA26 (transgenic for beta-galactosidase) mice. With a few exceptions, colonies showed either allblue cells or all clear cells after staining with X-Gal. Clonal growth was analyzed after 10-11 days in relation to cell density by determining colony size and plating efficiency. Growth was density dependent (no growth below 10,000 cell/ ml) and thus single cell cloning was notaccomplished. An average plating efficiency of 4% was found for EGF-responsive neural cells derived from day 15-18 murine embryos when cultured at 12,500 to 200,000 cells/ml. Similar results were obtained with1-day-old postnatal neural cells. When colonies were categorized by size, the relative number of colonies over 50 cells appeared to be maximum at 50,000 plated cells/ml. After 11 days in culture, 94, 96, and 78% of the colonies contained cells that expressed nestin, neurofilament, and GFAP, respectively. Double-label experiments revealed that > 62% of the colonies contained both GFAP and neurofilament expressing cells. These studies establish the existence of at least two populations of clonal neural progenitors: CFU-Ne and CFU-NeGl in fetal and postnatal murine brain. (C) 1997 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 08:10:12