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Titolo:
COMPARISON OF 5 INCUBATION SYSTEMS FOR RAT-LIVER SLICES USING FUNCTIONAL AND VIABILITY PARAMETERS
Autore:
OLINGA P; GROEN K; HOF IH; DEKANTER R; KOSTER HJ; LEEMAN WR; RUTTEN AAJJL; VANTWILLERT K; GROOTHUIS GMM;
Indirizzi:
UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOKINET & DRUG DELIVERY,GRONINGENINST DRUG STUDIES NL-9713 AV GRONINGEN NETHERLANDS RIVM,DEPT BIOTRANSFORMAT PHARMACO & TOXICOKINET NL-3270 BA BILTHOVEN NETHERLANDS SOLVAY DUPHAR LABS,DRUG SAFETY DEPT NL-1380 DA WEESP NETHERLANDS TNO,NUTR & FOOD RES INST,DIV TOXICOL NL-3700 AJ ZEIST NETHERLANDS
Titolo Testata:
Journal of pharmacological and toxicological methods
fascicolo: 2, volume: 38, anno: 1997,
pagine: 59 - 69
SICI:
1056-8719(1997)38:2<59:CO5ISF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYNAMIC ORGAN-CULTURE; CUT TISSUE-SLICES; ENZYME-ACTIVITIES; METABOLISM; HEPATOCYTES; ANTIPYRINE; CYTOCHROME-P450; HEPATOTOXICITY; TOXICOLOGY; INDUCTION;
Keywords:
RAT LIVER SLICES; INCUBATION SYSTEM; VIABILITY; DRUG METABOLISM;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
P. Olinga et al., "COMPARISON OF 5 INCUBATION SYSTEMS FOR RAT-LIVER SLICES USING FUNCTIONAL AND VIABILITY PARAMETERS", Journal of pharmacological and toxicological methods, 38(2), 1997, pp. 59-69

Abstract

Precision-cut liver slices are presently used for various research objects, e.g. to study metabolism, transport, and toxicity of xenobiotics. Various incubation systems are presently employed, but a systematiccomparison between these incubation systems with respect to preservation of slice function has not been performed yet. Therefore, we started a comparative study to evaluate five of these systems: the shaken flask (an Erlenmeyer in a shaking water bath), the stirred-well (24-wellculture plate equipped with grids and magnetic stirrers), rocker platform (6-well culture plate with Netwell insert rocked on a platform), the roller system (dynamic organ culture rolled on an insert in a glass vial), and the 6-well shaker (6-well culture plate in a shaking water bath). The liver slices were incubated in these incubation systems for 0.5, 1.5, and 24.5 h and subsequently subjected to viability and metabolic function tests. The viability of the incubated liver slices was evaluated by potassium content, MTT assay, energy charge, histomorphology, and LDH leakage. Their metabolic functions were studied by determination of the metabolism of lidocaine, testosterone, and antipyrine. Up to 1.5 h of incubation all five incubation systems gave similar results with respect to viability and metabolic function of the liver slices. However, after 24 h, the shaken flask, the rocker platform, andthe 6-well shaker incubation systems appeared to be superior to the stirred well and the roller incubation systems. (C) 1997 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 10:15:54