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Titolo:
INDUCTION OF C-KIT MOLECULES ON HUMAN CD34+ C-KIT(LESS-THAN-LOW) CELLS - EVIDENCE FOR CD34+/C-KIT(LESS-THAN-LOW) CELLS AS PRIMITIVE HEMATOPOIETIC STEM-CELLS/
Autore:
SOGO S; INABA M; OGATA H; HISHA H; ADACHI Y; MORI S; TOKI J; YAMANISHI K; KANZAKI H; ADACHI M; IKEHARA S;
Indirizzi:
KANSAI MED UNIV,DEPT PATHOL 1,10-15 FUMIZONO CHO MORIGUCHI OSAKA 570 JAPAN KANSAI MED UNIV,DEPT PATHOL 1 MORIGUCHI OSAKA 570 JAPAN KANSAI MED UNIV,DEPT PEDIAT MORIGUCHI OSAKA 570 JAPAN KANSAI MED UNIV,DEPT OBSTET & GYNECOL MORIGUCHI OSAKA 570 JAPAN OTSUKA PHARMACEUT CO LTD,CELLULAR TECHNOL INST TOKUSHIMA 77101 JAPAN
Titolo Testata:
Stem cells
fascicolo: 6, volume: 15, anno: 1997,
pagine: 420 - 429
SICI:
1066-5099(1997)15:6<420:IOCMOH>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
UMBILICAL-CORD BLOOD; COLONY-STIMULATING FACTOR; BONE-MARROW STROMA; PROGENITOR CELLS; IN-VITRO; CD34(+)CD38(-) CELLS; PERIPHERAL-BLOOD; EXPRESSION; PROLIFERATION; CD34+;
Keywords:
C-KIT; CD34; HUMAN CORD BLOOD; HEMATOPOIETIC STEM CELLS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
S. Sogo et al., "INDUCTION OF C-KIT MOLECULES ON HUMAN CD34+ C-KIT(LESS-THAN-LOW) CELLS - EVIDENCE FOR CD34+/C-KIT(LESS-THAN-LOW) CELLS AS PRIMITIVE HEMATOPOIETIC STEM-CELLS/", Stem cells, 15(6), 1997, pp. 420-429

Abstract

c-kit, a receptor for stem cell factor, has been widely accepted as adistinctive marker for hematopoietic stem cells, However, the level of c-kit expression on pluripotent hematopoietic stem cells is still controversial in mice and humans, We purified CD34(+)/c-kit(<low) cells (phenotypically c-kit-negative but only detectable at the message level) from human cord blood and examined their maturational steps in relation to the expression of c-kit molecules, When the CD34(+)/c-kit(<low) cells were cultured with cytokines (flt 3 ligand, interleukin 6 and interleukin 7) plus immobilized anti-CD34 monoclonal antibody (to crosslink CD34 molecules), c-kit molecules were clearly induced within 24 h, The c-kit expression gradually increased until day 8, When CD34(+)/c-kit(low) or CD34(+)/c-kit(+) cells that had been induced from CD34(+)/c-kit(<low) cells were resorted and recultured using a methylcellulose culture system, they showed the same colony-forming ability as the freshly isolated CD34(+)/c-kit(low) or CD34(+)/c-kit(+) cells, respectively, Furthermore, CD34(+)/c-kit(<low) cells have a similar hematopoietic potential to CD34(+)/c-kit(low) cells in assays for long-term culture initiating cell and colony-forming unit culture generated from long-term cultures, These findings suggest that CD34(+)/c-kit(<low) cells mature into CD34(+)/c-kit(low) and CD34(+)/c-kit(+) cells, and acquire the reactivity to various humoral hematopoietic stimuli, Moreover, CD34(+)/c-kit(<low) cells showed a low level of rhodamine 123 retention, suggesting that CD34(+)/c-kit(<low) cells have multidrug resistance, Therefore, the CD34(+)/c-kit(<low) cells without colony-forming nit-granulocyte-erythroid-macrophage-megakaryocyte activity are also a pluripotent hematopoietic stem cell population, and the expression of c-kit on c-kit(<low) cells is the first maturational step of hematopoiesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 08:17:01