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Titolo:
TIME-RESOLVED LUMINESCENCE AS A NOVEL DETECTION MODE FOR THE SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF GADOLINIUM-DOTA AND GD3+
Autore:
MOUTIEZ E; PROGNON P; BOURRINET P; ZEHAF S; DENCAUSSE A; MAHUZIER G;
Indirizzi:
FAC PHARM,LAB CHIM ANALYT 2,5 RUE JEAN BAPTISTE CLEMENT F-92290 CHATENAY MALABR FRANCE LAB GUERBET SA F-95943 ROISSY FRANCE
Titolo Testata:
Analyst
fascicolo: 11, volume: 122, anno: 1997,
pagine: 1347 - 1352
SICI:
0003-2654(1997)122:11<1347:TLAAND>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACID COMPLEX; SPECTROMETRY; FLUORESCENCE; EUROPIUM; TERBIUM; PLASMA; AGENT; URINE;
Keywords:
GADOLINIUM; GD-DOTA; HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; TIME-RESOLVED LUMINESCENCE;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
E. Moutiez et al., "TIME-RESOLVED LUMINESCENCE AS A NOVEL DETECTION MODE FOR THE SIMULTANEOUS HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF GADOLINIUM-DOTA AND GD3+", Analyst, 122(11), 1997, pp. 1347-1352

Abstract

The simultaneous determination in aqueous media of the gadolinium ion(Gd3+) and the macrocyclic chelate Gd-DOTA (DOTA = 1, 4, 7, 10-tetraazacyclododecane-1, 1, 4, 7, 10-tetraacetic acid) used in magnetic resonance imaging (MRI) is described, The proposed approach combines a reversed-phase high-performance liquid chromatographic (RP-HPLC) separation and time-resolved luminescence detection (TRL). The spectroscopic properties of Gd-DOTA and various other Gd chelates (Gd-CDTA, Gd-TETA, Gd-EDTA, Gd-DTPA and Gd-TTHA) (CDTA = cyclohexanediaminetetraacetic acid; TETA = 1, 4, 8, 11-tetraazacyclotetradecane-1, 4, 8, 11-tetraacetic acid; EDTA = ethylenediaminetetraacetic acid; DTPA = diethylenetriaminepentaacetic acid; TTHA = triethylenetetraaminehexaacetic acid) wereestablished in order to optimize TRL detection (lambda(exc) = 274 nm,lambda(em) = 313 nm, delay time 0.1 ms, gate time 10 ms), The Gd3+ ion possibly present in the matrix was detected as a Gd-CDTA chelate after dilution of the sample by a pH 7.6-buffered 1 x 10(-2) M aqueous CDTA solution, Then, Gd-DOTA and Gd3+ ion (as Gd-CDTA chelate) were chromatographed on an RP C-8 HPLC column, The eluent consisted of pH 7.6 TRIS-HCl buffer + acetonitrile (98 + 2 v/v), The analytical figures of merit obtained for Gd-DOTA in aqueous solution were as follows: linearity was assumed from 10 to 5000 mu mol l(-1); the limit of detection (LOD, S/N = 3) was 21 mu mol l(-1), which corresponds to 235 ng of Gd-DOTA injected, The repeatability (RSD = 10%, n = 15) and the intra-assay reproducibility(RSD = 12%, n = 6) were studied for a 50 mu mol l(-1)solution, With this method, 130 ng of Gd3+ ion (as Gd-CDTA) injected can be detected, which corresponds to an LOD of 42 mu mol l(-1), The LOQ fur Gd3+ ion was 140 mu mol l(-1). Consequently, Gd3+ ion can be distinguished from chelated Gd-DOTA in aqueous solution (at the 0.2% level).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 16:51:22