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Titolo:
ELEVATED EXPRESSION OF ETS-1 GENE IN A METASTATIC, TUMORIGENIC HUMAN PROSTATE EPITHELIAL-CELL LINE TRANSFORMED BY THE V-KI-RAS ONCOGENE
Autore:
CHEN ZQ; FISHER RJ; LI BQ; KAMATA T; KUNG HF; LAUTENBERGER JA; RHIM JS;
Indirizzi:
NCI,LAB BIOCHEM PHYSIOL,BLDG 567,RM 152 FREDERICK MD 21702 NCI,LAB BIOCHEM PHYSIOL FREDERICK MD 21702 NCI,LAB GENOM DIVERS FREDERICK MD 21702 NCI,FREDERICK CANC RES & DEV CTR,IRSP,SAIC FREDERICK FREDERICK MD 21702
Titolo Testata:
International journal of oncology
fascicolo: 6, volume: 11, anno: 1997,
pagine: 1179 - 1184
SICI:
1019-6439(1997)11:6<1179:EEOEGI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR; MUTATIONS; C-ETS1; TRANSACTIVATION; ACTIVATION; CARCINOMA; PROTEINS; FAMILY; CANCER; DNA;
Keywords:
PROSTATE; ETS-1 GENE; METASTASIS; V-KI-RAS; EPITHELIAL CELLS;
Tipo documento:
Article
Natura:
Periodico
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
Z.Q. Chen et al., "ELEVATED EXPRESSION OF ETS-1 GENE IN A METASTATIC, TUMORIGENIC HUMAN PROSTATE EPITHELIAL-CELL LINE TRANSFORMED BY THE V-KI-RAS ONCOGENE", International journal of oncology, 11(6), 1997, pp. 1179-1184

Abstract

A suitable in vitro model system to investigate mechanisms of human prostate carcinogenesis is much needed. We have previously demonstratedthat an immortal, but non-tumorigenic, human prostate epithelial cellline (267B(1)) can be malignantly transformed by the v-Ki-ras oncogene, and it can serve as a useful model for investigation of the progression steps of prostate carcinogenesis. In this study, we report for the first time the invasive/metastatic phenotype of the v-Ki-ras transformed 267B, cells (267B(1)/Ki-ras). In addition, comparing non-tumorigenic 267B, and metastatic tumorigenic 267B(1)/Ki-ras human prostate epithelial cell lines, we have found that expression of ETS-1 and ERGB mRNA was elevated to 2-5 fold in the metastatic and tumorigenic 267B(1)/Ki-ras cell line. A specific ETS-1 monoclonal antibody E44 also revealed that the expression of ETS-1 protein level in 267B(1)/Ki-ras cell line was higher than those in 267B, cell line. However, other members of the ETS gene family such as ETS-2, GABP alpha and their mRNA expression levels were similar in both cell lines. The activation of MAP kinase, a downstream target for Ki-ras, was also shown. The expression of urokinase plasminogen activator (u-PA) was also increased in the metastatic 267B(1)/Ki-ras line. An obvious capability of invasion was observed in the 267B(1)/Ki-ras cell line, but not in the 267B(1) line usingBioCoat Matrigel invasion chamber assay system. The present study hasprovided evidence that the v-Ki-ras oncogene may activate the nucleartarget gene, ETS-1 gene, to mediate tumorigenic and metastatic capacity of the v-Ki-ras transformed prostate epithelial cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 16:32:31