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Titolo:
Production and functional characterization of recombinant bovine interleukin-8 as a specific neutrophil activator and chemoattractant
Autore:
Caswell, JL; Middleton, DM; Gordon, JR;
Indirizzi:
Univ Saskatchewan, Western Coll Vet Med, Dept Vet Pathol, Saskatoon, SK S7N Univ Saskatchewan Saskatoon SK Canada S7N 5B4 t Pathol, Saskatoon, SK S7N UnivS7Nskatchewan, Western Coll Vet Med, Dept Vet Microbiol, Saskatoon, SKUniv Saskatchewan Saskatoon SK Canada S7N 5B4 et Microbiol, Saskatoon, SK
Titolo Testata:
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
fascicolo: 4, volume: 67, anno: 1999,
pagine: 327 - 340
SICI:
0165-2427(19990301)67:4<327:PAFCOR>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN EOSINOPHILS; IN-VITRO; EXPRESSION; CYTOKINES; MIGRATION; CLONING;
Keywords:
polymorphonuclear leukocytes; cytokines; cattle; chemokines; chemotactic factors; recombinant proteins;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
22
Recensione:
Indirizzi per estratti:
Indirizzo: Caswell, JL Univ Guelph, Hlth Anim Lab, POB 3612, Guelph, ON N1H 6R8, Canada Univ Guelph POB 3612 Guelph ON Canada N1H 6R8 N1H 6R8, Canada
Citazione:
J.L. Caswell et al., "Production and functional characterization of recombinant bovine interleukin-8 as a specific neutrophil activator and chemoattractant", VET IMMUNOL, 67(4), 1999, pp. 327-340

Abstract

Interleukin-8, a member of the chemokine family of cytokines, is a potent neutrophil chemoattractant in many non-rodent species. In this study, recombinant bovine interleukin-8 (rbIL-8) was expressed in bacteria as a glutathione-S-transferase fusion protein. The fusion protein was purified by glutathione-Sepharose affinity chromatography and recombinant rbIL-8 was eluted by cleaving with thrombin. The purified rbIL-8 molecule was approximately 8kDa and was confirmed as authentic IL-8 by Western analysis. Recombinant bovine IL-8 induced specific dose-dependent in vitro chemotaxis of neutrophils at doses as low as 1.0 ng/ml, and this activity was inhibited by pre-treatment of rbIL-8 with a monoclonal antibody to ovine IL-8. Neutrophils exposed to rbIL-8 developed pseudopodia and became elongated as determined by microscopic analysis and flow cytometry. Injection of 3.3 ng to 3.3 mu g of rbIL-8 into the skin of a normal calf induced dose-dependent recruitment ofneutrophils but not eosinophils. Intravascular margination of neutrophils was obvious at the injection sites from 15 to 60 min after administration of rbIL-8, and extravascular neutrophil numbers increased steadily from 1 to18 h after injection. Neutrophils with morphologic features of apoptosis were detected in these lesions at 18 and 30 h after injection, and this correlated with reduction in the number of dermal neutrophils, These results confirm unequivocally that bovine IL-8 functions as a neutrophil, but not an eosinophil, chemoattractant in vitro and in vivo. (C) 1999 Elsevier ScienceB.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:36:07