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Titolo:
HGF activates signal transduction from EPO receptor on human cord blood CD34(+)/CD45(+) cells
Autore:
Iguchi, T; Sogo, S; Hisha, H; Taketani, S; Adachi, Y; Miyazaki, R; Ogata, H; Masuda, S; Sasaki, R; Ito, M; Fukuhara, S; Ikehara, S;
Indirizzi:
Kansai Med Univ, Dept Pathol 1, Moriguchi, Osaka 5708506, Japan Kansai MedUniv Moriguchi Osaka Japan 5708506 guchi, Osaka 5708506, Japan Kansai Med Univ, Dept Hyg, Moriguchi, Osaka 570, Japan Kansai Med Univ Moriguchi Osaka Japan 570 yg, Moriguchi, Osaka 570, Japan Kansai Med Univ, Dept Pediat, Moriguchi, Osaka 570, Japan Kansai Med UnivMoriguchi Osaka Japan 570 at, Moriguchi, Osaka 570, Japan Kansai Med Univ, Dept Internal Med 1, Moriguchi, Osaka 570, Japan Kansai Med Univ Moriguchi Osaka Japan 570 1, Moriguchi, Osaka 570, Japan Otsuka Pharmaceut Co Ltd, Cellular Technol Inst, Tokushima 77101, Japan Otsuka Pharmaceut Co Ltd Tokushima Japan 77101 t, Tokushima 77101, Japan Kyoto6,niv, Grad Sch Agr, Div Appl Sci, Lab Biosignals & Response, Kyoto 60 Kyoto Univ Kyoto Japan 606 Appl Sci, Lab Biosignals & Response, Kyoto 60
Titolo Testata:
STEM CELLS
fascicolo: 2, volume: 17, anno: 1999,
pagine: 82 - 91
SICI:
1066-5099(1999)17:2<82:HASTFE>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEPATOCYTE GROWTH-FACTOR; BONE-MARROW PLASMA; SCATTER FACTOR; ERYTHROID PROGENITORS; MET PROTOONCOGENE; MULTIPLE-MYELOMA; CANCER PATIENTS; C-MET; ERYTHROPOIETIN; EXPRESSION;
Keywords:
HGF; human cord blood; signal transduction; erythropoiesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Ikehara, S Kansai6,ed Univ, Dept Pathol 1, 10-15 Fumizono Cho, Moriguchi, Osaka 570850 Kansai Med Univ 10-15 Fumizono Cho Moriguchi Osaka Japan 5708506
Citazione:
T. Iguchi et al., "HGF activates signal transduction from EPO receptor on human cord blood CD34(+)/CD45(+) cells", STEM CELLS, 17(2), 1999, pp. 82-91

Abstract

Hepatocyte growth factor (HGF) is a multifunctional cytokine with early hematopoiesis-stimulatory activity. Here, we focus on its erythropoiesis-stimulatory effect on highly purified human hematopoietic progenitor cells (CD34(+)/CD45(+) cells) derived from the cord blood. In immunoblot analyses, c-met protein (a receptor of HGF) was detected in the CD34(+)/CD45(+) cells, although the expression levels were different among samples. The c-met expression was facilitated by incubation of the cells with stem cell factor (SCF) or interleukin 3 (IL-3), even if the expression level had been low, IL-6, G-CSF, or erythropoietin (EPO) did not show such a stimulatory effect on the c-met expression of the cells. When HGF was added to the CD34(+)/CD45(+) cells in the presence of SCF, the numbers of CD36(+)/CD11b(-) cells (very early erythroid lineage cells) and BFU-E increased. EPO-dependent tyrosine phosphorylation of Stat 5 also increased, but the EPO receptor (EPO-R) expression remained unchanged in the CD34(+)/CD45(+) cells treated with SCF + HGF, Our present study suggests that stimulationof the HGF/c-met signal is concomitant with induction of c-met protein by SCF, The subsequent enhancement of signal transduction via the activation of Stat 5 from the EPO-R plays a crucial role in the commitment of hematopoietic stem cells into erythroid lineage cells.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 16:46:01