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Titolo:
Distinct biological effects of macrophage inflammatory protein-1 alpha andstroma-derived factor-1 alpha on CD34(+) hemopoietic cells
Autore:
Durig, J; Testa, NG; Heyworth, CM;
Indirizzi:
Christie&Hosp NHS Trust, Paterson Inst Canc Res, CRC, Sect Haemopoiet CellChristie Hosp NHS Trust Manchester Lancs England M20 4BX Haemopoiet Cell Univ Hosp Essen, Dept Haematol, Essen, Germany Univ Hosp Essen Essen Germany Hosp Essen, Dept Haematol, Essen, Germany
Titolo Testata:
STEM CELLS
fascicolo: 2, volume: 17, anno: 1999,
pagine: 62 - 71
SICI:
1066-5099(1999)17:2<62:DBEOMI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC PROGENITOR CELLS; INTERFERON-INDUCIBLE PROTEIN-10; GROWTH-FACTOR STIMULATION; C CHEMOKINE RECEPTOR; MOLECULAR-CLONING; FUNCTIONAL EXPRESSION; KINASE-ACTIVITY; HUMAN MONOCYTES; LINE; MIP-1-ALPHA;
Keywords:
chemokines; MIP-1 alpha; SDF-1 alpha; hemopoietic cells; calcium flux; growth inhibition; adhesion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Heyworth, CM Christie&Hosp NHS Trust, Paterson Inst Canc Res, CRC, Sect Haemopoiet Cell Christie Hosp NHS Trust Manchester Lancs England M20 4BX ell
Citazione:
J. Durig et al., "Distinct biological effects of macrophage inflammatory protein-1 alpha andstroma-derived factor-1 alpha on CD34(+) hemopoietic cells", STEM CELLS, 17(2), 1999, pp. 62-71

Abstract

Chemokines are important regulators of both hemopoietic progenitor cell (HPC) proliferation and adhesion to extracellular matrix molecules, Here, we compared the biological effects of the CC chemokine macrophage inflammatoryprotein-1 alpha (MIP-1 alpha) with those of the CXC chemokine stroma-derived factor-1 alpha (SDF-1 alpha) on immunomagnetically purified CD34(+) cells from leukapheresis products (LP CD34(+)), In particular, studies on chemokine-induced alterations of LP CD34(+) cell attachment to fibronectin-coated plastic surfaces, proliferation of these cells in colony-forming cell (CFC) assays and intracellular calcium mobilization were performed, MIP-1 alpha but not SDF-1 alpha was found to increase the adhesion of LP CD34(+) cells to fibronectin in a dose-dependent manner. Both chemokines elicited growth-suppressive effects on LP CD34(+) cells in CFC assays, While MIP-la reduced the number of granulomonocytic (CFC-GM) and erythroid (BFU-E) colonies to the same extent, SDF-1 alpha showed a significantly greater inhibitory effect on CFC-GM than BFU-E, Finally, we demonstrated that SDF-1 alpha but not MIP-1 alpha triggers increases in intracellular calcium in LP CD34(+) cells. The SDF-1 alpha-induced calcium response was rapid and concentration-dependent, with a maximal stimulation observed at greater than or equal to 15ng/ml, In conclusion, our data suggest distinct biological properties of SDF-1 alpha and MIP-1 alpha in terms of modulation of LP CD34(+) cell adhesion to fibronectin and intracellular calcium levels, However, comparable growth-suppressive effects on HPC proliferation were observed, indicating thatthis feature may be independent of chemokine-induced calcium responses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 05:13:33