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Titolo:
A genetic approach to trace neural circuits
Autore:
Horowitz, LF; Montmayeur, JP; Echelard, Y; Buck, LB;
Indirizzi:
Harvard2115v, Sch Med, Howard Hughes Med Inst, Dept Neurobiol, Boston, MA 0 Harvard Univ Boston MA USA 02115 s Med Inst, Dept Neurobiol, Boston, MA 0 Genzyme Corp, Framingham, MA 01701 USA Genzyme Corp Framingham MA USA 01701 nzyme Corp, Framingham, MA 01701 USA
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 6, volume: 96, anno: 1999,
pagine: 3194 - 3199
SICI:
0027-8424(19990316)96:6<3194:AGATTN>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-NEURONAL TRANSPORT; OLFACTORY MARKER PROTEIN; HORSERADISH-PEROXIDASE; ZNSO4 IRRIGATION; WGA-HRP; RAT; EXPRESSION; EPITHELIUM; SYSTEM; SYNAPTOPHYSIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Buck, LB Harvard Univ, Sch Med, Howard Hughes Med Inst, Dept Neurobiol, 230 Longwood Harvard Univ 230 Longwood Ave Boston MA USA 02115 l, 230 Longwood
Citazione:
L.F. Horowitz et al., "A genetic approach to trace neural circuits", P NAS US, 96(6), 1999, pp. 3194-3199

Abstract

Mammalian nervous system function involves billions of neurons which are interconnected in a multitude of neural circuits. Here we describe a geneticapproach to chart neural circuits. By using an olfactory-specific promoter, H-e selectively expressed barley lectin In sensory neurons in the olfactory epithelium and vomeronasal organ of transgenic mice. The lectin was transported through the axons of those neurons to the olfactory bulb, transferred to the bulb neurons with which they synapse. and transported through theaxons of bulb neurons to the olfactory cortex, The lectin also was retrogradely transported from the bulb to neuromodulatory brain areas,;No evidencecould be obtained for adverse effects of the lectin on odorant receptor gene expression, sensory axon targeting in the bulb, or the generation or transmission of signals by olfactory sensory neurons. Transneuronal transfer was detected prenatally in the odor-sensing pathway, but only postnatally inthe pheromone-sensing pathway, suggesting that odors, but not pheromones, may be sensed in utero, Our studies demonstrate that a plant lectin can serve as a transneuronal tracer when its expression is genetically targeted toa subset of neurons. This technology can potentially be applied to a variety of vertebrate and invertebrate neural systems and may be particularly valuable for mapping connections formed by small subsets of neurons and for studying the development of connectivity as it occurs in utero.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:16:37