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Titolo:
Characterisation of kainate receptor mediated whole-cell currents in rat cultured cerebellar granule cells
Autore:
Savidge, JR; Sturgess, NC; Bristow, DR; Lock, EA;
Indirizzi:
Univlandchester, Sch Biol Sci, Div Neurosci, Manchester M13 9PT, Lancs, Eng Univ Manchester Manchester Lancs England M13 9PT ster M13 9PT, Lancs, Eng Zeneca, Cent Toxicol Lab, Macclesfield SK10 4TJ, Cheshire, England ZenecaMacclesfield Cheshire England SK10 4TJ SK10 4TJ, Cheshire, England
Titolo Testata:
NEUROPHARMACOLOGY
fascicolo: 3, volume: 38, anno: 1999,
pagine: 375 - 382
SICI:
0028-3908(199903)38:3<375:COKRMW>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTAMATE RECEPTORS; HIPPOCAMPAL-NEURONS; ALPHA-AMINO-3-HYDROXY-5-METHYL-4-ISOXAZOLE PROPIONATE; PREFERRING RECEPTORS; AMPA; CHANNELS; SUBUNITS; DESENSITIZATION; 2,3-BENZODIAZEPINES; RECTIFICATION;
Keywords:
AMPA receptor; kainate receptor; GYKI 53655; (2S,4R)-4-methyl glutamate; cyclothiazide; concanavalin A; cerebellar granule cell;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Savidge, JR Univ Manchester, Sch Biol Sci, Div Neurosci, 1-124 Stopford Bldg,Oxford Rd, Univ Manchester 1-124 Stopford Bldg,Oxford Rd Manchester Lancs England M13 9PT
Citazione:
J.R. Savidge et al., "Characterisation of kainate receptor mediated whole-cell currents in rat cultured cerebellar granule cells", NEUROPHARM, 38(3), 1999, pp. 375-382

Abstract

Whole-cell voltage clamp recordings have been used to identify and characterise inward currents mediated by native kainate receptors in rat cultured cerebellar granule cells. While the selective AMPA receptor antagonist GYKI53655 (50 mu M) completely abolished inward currents evoked by AMPA (10-100 mu M) in the presence of cyclothiazide (100 mu M), kainate evoked currents in cells pretreated with concanavalin A (Con A) always showed a component(35-140 pA, n = 13) resistant to blockade. The majority (73 +/- 7%, it = 5) of GYKI 53655-resistant kainate-evoked inward currents remained in the presence of 100 mu M AMPA. However, these currents were reversibly blocked bythe competitive AMPA/kainate receptor antagonist NBQX (100 mu M). (2S, 4R)-4-methylglutamate (SYM 2081, 10 mu M) evoked inward currents in Con A treated cells (15-60 pA, n = 7), which were resistant to complete blockade by GYKI 53655 (50 mu M) but antagonised by NBQX (100 mu M). Kainate-evoked responses in the presence of GYKI 53655 (50 mu M) had linear or slightly outwardly rectifying current-voltage (I-V) relationships in all cells examined (n= 5) and were resistant to blockade by Joro spider toxin (JsTx, 1 mu M; n = 5). These results provide evidence that rat cultured cerebellar granule cells express functional kainate receptors made up of subunits which are edited at the Q/R site, and that SYM 2081 is an agonist at these native kainate receptors with a greater selectivity than kainate itself. (C) 1999 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/07/20 alle ore 01:50:20