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Titolo:
Chromatin assembly in yeast cell-free extracts
Autore:
Schultz, MC;
Indirizzi:
Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada Univ Alberta Edmonton AB Canada T6G 2H7 hem, Edmonton, AB T6G 2H7, Canada
Titolo Testata:
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY
fascicolo: 2, volume: 17, anno: 1999,
pagine: 161 - 172
SICI:
1046-2023(199902)17:2<161:CAIYCE>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
CASEIN KINASE-II; SACCHAROMYCES-CEREVISIAE; DNA; REPLICATION; PROTEIN; RNA; BINDING; SYSTEM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Schultz, MC Univ Alberta, Dept Biochem, Edmonton, AB T6G 2H7, Canada Univ Alberta Edmonton AB Canada T6G 2H7 n, AB T6G 2H7, Canada
Citazione:
M.C. Schultz, "Chromatin assembly in yeast cell-free extracts", METHODS, 17(2), 1999, pp. 161-172

Abstract

A simple method for preparing chromatin assembly extracts has not been available for budding yeast. Here I describe such a method in detail. The assembly extract, a crude 100,000g supernatant, is prepared from cells disrupted in a manual or motorized grinder while they are frozen. The core histonesand all soluble protein factors required for chromatin assembly under physiological conditions are present in the extract. Assembly is sensitive to mutation of lysine residues in the aminoterminal tail of histone H4 whose acetylation is associated with nucleosome deposition in vivo. The reaction isATP dependent, and assembly-driven DNA supercoiling occurs with the same efficiency as in extracts from mammalian somatic cells. This simple system offers a unique opportunity to analyze chromatin metabolism by a combined biochemical and genetic approach that is not feasible for any other model organism. (C) 1999 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 07:02:09