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Titolo:
Detection of bovine retrospumavirus by the polymerase chain reaction
Autore:
Pamba, R; Jeronimo, C; Archambault, D;
Indirizzi:
UnivCanadac, Dept Biol Sci, Lab Mol Virol & Immunol, Montreal, PQ H3C 3P8,Univ Quebec Montreal PQ Canada H3C 3P8 l & Immunol, Montreal, PQ H3C 3P8,
Titolo Testata:
JOURNAL OF VIROLOGICAL METHODS
fascicolo: 1-2, volume: 78, anno: 1999,
pagine: 199 - 208
SICI:
0166-0934(199903)78:1-2<199:DOBRBT>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN FOAMY VIRUS; IMMUNODEFICIENCY-LIKE VIRUS; MULTIPLE RETROVIRAL INFECTIONS; SYNCYTIAL VIRUS; TRANSGENIC MICE; LEUKEMIA-VIRUS; GENE-EXPRESSION; ENVELOPE GENE; CATTLE; DNA;
Keywords:
bovine retrospumavirus; provirus DNA detection; polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Archambault, D UnivrQuebec, Dept Biol Sci, Lab Mol Virol & Immunol, POB 8888,Succursale Ct Univ Quebec POB 8888,Succursale Ctr Ville Montreal PQ Canada H3C 3P8
Citazione:
R. Pamba et al., "Detection of bovine retrospumavirus by the polymerase chain reaction", J VIROL MET, 78(1-2), 1999, pp. 199-208

Abstract

A polymerase chain reaction (PCR) assay was developed for detection of bovine retrospumavirus (bovine syncytial virus; BSV) provirus DNA. Two different sets of oligonucleotide primers complementary to sequences located in the gag and the pol/env gene regions were used and compared for their abilityto amplify the targeted BSV sequences by PCR. The results obtained from this study have shown that it is possible to amplify the BSV provirus DNA sequences not only from total DNA of BSV-infected cell cultures, but also fromtotal DNA of various tissues and peripheral blood mononuclear cells (PBMCs) that were collected from two rabbits experimentally infected with BSV. Sensitivities of the PCR for amplification of BSV gag and pol/env nucleic acid sequences from cell culture total DNA were 10 ng and 10 pg of DNA, respectively, as determined by the analysis of the amplified PCR products on ethidium bromide-stained agarose gels. The specificity of the PCR for both primer sets tested was confirmed when the amplified cDNA products of the expected size reacted positively with the corresponding virus-specific digoxigenin-labeled cDNA probes in Southern blot chemiluminescent hybridization assays. No amplification was obtained when the BSV-specific primers were used inthe PCR with DNA material specific to either bovine leukemia Virus (BLV) or bovine immunodeficiency virus (BIV) provirus genomic DNA. No cross-hybridization was obtained when the BSV-specific cDNA probes were allowed to react with BLV or BIV provirus DNA. The PCR targeting the gag and pol/env gene regions of the BSV provirus genome may be an alternative to conventional methods for the confirmation of the presence of BSV in cell cultures used forvirus isolation, and for the diagnosis of BSV infection from bovine peripheral blood leukocytes. (C) 1999 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:42:32