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Titolo:
DEVELOPMENT OF A REVERSE-TRANSCRIPTASE PCR-ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA IN PLASMA - COMPARISON WITH COMMERCIAL QUANTITATIVE ASSAYS
Autore:
TRABAUD MA; AUDOLY G; LERICHE K; COTTE L; RITTER J; SEPETJAN M; TREPO C;
Indirizzi:
INSERM,U271,151 COURS ALBERT THOMAS F-69424 LYON 03 FRANCE INSERM,U271 F-69424 LYON 03 FRANCE HOP HOTEL DIEU,SERV HEPATOGASTROENTEROL F-69002 LYON FRANCE FAC MED,HYG LAB F-69373 LYON 08 FRANCE
Titolo Testata:
Journal of clinical microbiology
fascicolo: 5, volume: 35, anno: 1997,
pagine: 1251 - 1254
SICI:
0095-1137(1997)35:5<1251:DOARPI>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE CHAIN-REACTION; HIV-1; INFECTION; DNA; VIREMIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
M.A. Trabaud et al., "DEVELOPMENT OF A REVERSE-TRANSCRIPTASE PCR-ENZYME-LINKED IMMUNOSORBENT-ASSAY FOR QUANTIFICATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 RNA IN PLASMA - COMPARISON WITH COMMERCIAL QUANTITATIVE ASSAYS", Journal of clinical microbiology, 35(5), 1997, pp. 1251-1254

Abstract

A quantitative reverse transcriptase PCR assay with automated detection by nonradioactive hybridization was developed for the determinationof human immunodeficiency virus (HIV) type 1 RNA levels. This assay is based on the use of an external standard curve with an internal standard. The accuracy of quantification was verified by comparison with reference commercial tests, the Chiron branched-DNA and Roche AMPLICOR HIV MONITOR assays. This assay was able to quantify viremia in patients with CD4 cell numbers below and above 500/mm(3) and to quantify someHIV strains which could not be titrated by the MONITOR assay.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/09/20 alle ore 13:55:31