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Titolo:
MEASUREMENT OF LEVELS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE (RT) AND RT ACTIVITY-BLOCKING ANTIBODY IN HUMAN SERUM BYA NEW STANDARDIZED COLORIMETRIC ASSAY
Autore:
AWAD RJK; CORRIGAN GE; EKSTRAND DHL; THORSTENSSON R; KALLANDER CFR; GRONOWITZ JS;
Indirizzi:
CAVIDI TECH AB,UPPSALA SCI PK S-75183 UPPSALA SWEDEN CAVIDI TECH AB S-75183 UPPSALA SWEDEN UPPSALA UNIV,RES UNIT REPLICAT ENZYMOL,DEPT MED GENET UPPSALA SWEDEN SWEDISH INST INFECT DIS CONTROL STOCKHOLM SWEDEN
Titolo Testata:
Journal of clinical microbiology
fascicolo: 5, volume: 35, anno: 1997,
pagine: 1080 - 1089
SICI:
0095-1137(1997)35:5<1080:MOLOHT>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
INHIBITING ANTIBODY; DISEASE PROGRESSION; HIV; INFECTION; SUBTYPE; ASSOCIATION; TESTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
28
Recensione:
Indirizzi per estratti:
Citazione:
R.J.K. Awad et al., "MEASUREMENT OF LEVELS OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REVERSE-TRANSCRIPTASE (RT) AND RT ACTIVITY-BLOCKING ANTIBODY IN HUMAN SERUM BYA NEW STANDARDIZED COLORIMETRIC ASSAY", Journal of clinical microbiology, 35(5), 1997, pp. 1080-1089

Abstract

Standardization and calibration of a new colorimetric assay for detection of reverse transcriptase (RT) was carried out for optimal detection of RT activity-blocking antibody (RTb-Ab) in serum. A total of 99 of 100 Swedish and 54 of 54 African human immunodeficiency virus type 1(HIV-1) antibody-positive individuals had RTb-Ab. The one RTb-Ab-negative HIV-1 serum sample from a Swedish individual was obtained early during seroconversion. Five of 615 HIV-1-negative sera from tumor patients, pregnant women, patients undergoing routine viral diagnostics, and blood donors gave false-positive results, In addition, 3 of 126 HIV-1-negative African serum samples and 2 of 91 serum samples selected because of false reactivity in other commercially available HIV antibodyassays were positive for RTb-Ab. RT activity and RTb-Ab were measuredin sera from newly HIV-1-infected individuals during seroconversion. Peak RT activity was usually detected between days 8 and 13 after the onset of symptoms of primary infection. In addition, HIV-1 RTb-Ab was detected in the same recently infected individuals in most cases within 1 month and in some cases as early as 10 to 12 days after the onset of symptoms. A cross-reactivity study involving HIV-1 and HIV-2 RTb-Gbs and their homologous RT showed HIV-1 RTb-Ab to be highly type specific. None of 10 serum samples from HIV-l-infected individuals showed cross-reacting RTb-Ab toward HIV-2 RT, whereas 4 of 10 serum samples from HIV-2-infected patients showed cross-reactivity toward HIV-1 RT; however, the cross-reactivity toward HIV-1 RT was 3,000 times lower than that toward its homologous RT. Future uses for the assay with reference to the recent World Health Organization proposal for other methods instead of Western blotting (immunoblotting) for confirming HIV-1 infection and for methods for the diagnosis of infection as follow-up in vaccine trials are also discussed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/12/20 alle ore 16:15:45