Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
PURIFICATION AND MOLECULAR-CLONING OF SH2-CONTAINING AND SH3-CONTAINING INOSITOL POLYPHOSPHATE-5-PHOSPHATASE, WHICH IS INVOLVED IN THE SIGNALING PATHWAY OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR, ERYTHROPOIETIN, AND BCR-ABL
Autore:
ODAI H; SASAKI K; IWAMATSU A; NAKAMOTO T; UENO H; YAMAGATA T; MITANI K; YAZAKI Y; HIRAI H;
Indirizzi:
UNIV TOKYO,FAC MED,DEPT INTERNAL MED 3,BUNKYO KU,7-3-1 HONGO TOKYO 113 JAPAN UNIV TOKYO,FAC MED,DEPT INTERNAL MED 3,BUNKYO KU TOKYO 113 JAPAN KIRIN BREWERY CO LTD,CENT LAB KEY TECHNOL YOKOHAMA KANAGAWA JAPAN
Titolo Testata:
Blood
fascicolo: 8, volume: 89, anno: 1997,
pagine: 2745 - 2756
SICI:
0006-4971(1997)89:8<2745:PAMOSA>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-RECEPTOR; TYROSINE-PHOSPHORYLATED PROTEIN; PHOSPHOTYROSINE INTERACTION DOMAIN; NUCLEOTIDE EXCHANGE FACTOR; SRC HOMOLOGY-3 DOMAINS; CELL ANTIGEN RECEPTOR; GRB2 ADAPTER PROTEIN; C-CBL PROTOONCOGENE; HEMATOPOIETIC-CELLS; HUMAN-PLATELETS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
71
Recensione:
Indirizzi per estratti:
Citazione:
H. Odai et al., "PURIFICATION AND MOLECULAR-CLONING OF SH2-CONTAINING AND SH3-CONTAINING INOSITOL POLYPHOSPHATE-5-PHOSPHATASE, WHICH IS INVOLVED IN THE SIGNALING PATHWAY OF GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR, ERYTHROPOIETIN, AND BCR-ABL", Blood, 89(8), 1997, pp. 2745-2756

Abstract

Grb2/Ash and Shc are the adapter proteins that link tyrosine-kinase receptors to Ras and make tyrosine-kinase functionally associated with receptors and pas in fibroblasts and hematopoietic cells. Grb2/Ash andShc have the SH3, SH2, or phosphotyrosine binding domains. These domains bind to proteins containing proline-rich regions or tyrosine-phosphorylated proteins and contribute to the association of Grb2/Ash and She with other signaling molecules. However, there could remain unidentified signaling molecules that physically and functionally interact with these adapter proteins and have biologically important roles in thesignaling pathways, By using the GST fusion protein including the full length of Grb2/Ash, we have found that c-Cbl and an unidentified 135-kD protein (pp135) are associated with Grb2/Ash. We have also found that they become tyrosine-phosphorylated by treatment of a human leukemia cell line, UT-7, with granulocyte-macrophage colony-stimulating factor (GM-CSF). We have purified the pp135 by using GST-Grb2/Ash affinity column and have isolated the full-length complementary DNA (cDNA) encoding the pp135 using a cDNA probe, which was obtained by the degenerate polymerase chain reaction based on a peptide sequence of the purified pp135. The cloned cDNA has 3,958 nucleotides that contain a singlelong open reading frame of 3,567 nucleotides, encoding a 1,189 amino acid protein with a predicted molecular weight of approximately 133 kD. The deduced amino acid sequence reveals that pp135 is a protein thathas one SH2, one SH3, and one proline-rich domain. The pp135, which contains two motifs conserved among the inositol polyphosphate-5-phosphatase proteins, was shown to have the inositol polyphosphate-5-phosphatase activity. The pp135 was revealed to associate constitutively withGrb2/Ash and inducibly with Shc using UT-7 cells stimulated with GM-CSF. In the cell lines derived from human chronic myelogenous leukemia,pp135 was constitutively tyrosine-phosphorylated and associated with Shc and Bcr-Abl. These facts suggest that pp135 is a signaling molecule that has a unique enzymatic activity and should play an important role in the signaling pathway triggered by GM-CSF and in the transformation of hematopoietic cells caused by Bcr-Abl. (C) 1997 by The AmericanSociety of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 02:13:58