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Titolo:
THE CHARACTERIZATION, MOLECULAR-CLONING, AND EXPRESSION OF A NOVEL HEMATOPOIETIC-CELL ANTIGEN FROM CD34(-MARROW CELLS() HUMAN BONE)
Autore:
UCHIDA N; YANG Z; COMBS J; POURQUIE O; NGUYEN M; RAMANATHAN R; FU J; WELPLY A; CHEN S; WEDDELL G; SHARMA AK; LEIBY KR; KARAGOGEOS D; HILL B; HUMEAU L; STALLCUP WB; HOFFMAN R; TSUKAMOTO AS; GEARING DP; PEAULT B;
Indirizzi:
SYSTEMIX INC,3155 PORTER DR PALO ALTO CA 94304 CNRS,INST EMBRYOL CELLULAIRE & MOL,UPR 9064 F-94736 NOGENT SUR MARNE FRANCE INST MOL BIOL & BIOTECHNOL IRAKLION CRETE GREECE LA JOLLA CANC RES FDN LA JOLLA CA 92037
Titolo Testata:
Blood
fascicolo: 8, volume: 89, anno: 1997,
pagine: 2706 - 2716
SICI:
0006-4971(1997)89:8<2706:TCMAEO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGENITOR CELLS; IMMUNOGLOBULIN SUPERFAMILY; ADHESION MOLECULE; STEM-CELLS; T-CELLS; B-CELLS; RHODAMINE-123; GLYCOPROTEIN; SEPARATION; POPULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
N. Uchida et al., "THE CHARACTERIZATION, MOLECULAR-CLONING, AND EXPRESSION OF A NOVEL HEMATOPOIETIC-CELL ANTIGEN FROM CD34(-MARROW CELLS() HUMAN BONE)", Blood, 89(8), 1997, pp. 2706-2716

Abstract

The adhesion molecule BEN/SC1/DM-GRASP (BEN) is a marker in the developing chicken nervous system that is also expressed on the surface of embryonic and adult hematopoietic cells such as immature thymocytes, myeloid progenitors, and erythroid progenitors. F84.1 and KG-CAM, two monoclonal antibodies to rat neuronal glycoproteins with similarity to BEN, cross-react with an antigen on rat hematopoietic progenitors, butF84.1 only also recognizes human blood cell progenitors. We have defined the antigen recognized by F84.1 as the hematopoietic cell antigen (HCA). HCA expression was detected on 40% to 70% of CD34(+) fetal and adult bone marrow cells and mobilized peripheral blood cells, Precursor cell activity for long-term in vitro bone marrow cell culture was confined to the subset of CD34(+) cells that coexpress HCA. HCA is expressed by the most primitive subsets of CD34(+) cells, including all rhodamine 123(lo), Thy-1(+), and CD38(-/lo) CD34(+) adult bone marrow cells. HCA was also detected on myeloid progenitors but not on early B-cell progenitors. We also describe here the cloning and characterizationof cDNAs encoding two variants of the human HCA antigen (huHCA-1 and huHCA-2) and of a cDNA clone encoding rat HCA (raHCA). The deduced amino acid sequences of huHCA and raHCA are homologous to that of chickenBEN. Recombinant proteins produced from either human or rat HCA cDNAswere recognized by F84.1, whereas rat HCA but not human HCA was recognized by antirat KG-CAM. Expression of either form of huHCA in CHO cells conferred hemophilic adhesion that could be competed with soluble recombinant huHCA-Fc. The molecular cloning of HCA and the availabilityof recombinant HCA should permit further evaluation of its role in human and rodent hematopoiesis. (C) 1997 by The American Society of Hematology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/06/20 alle ore 08:30:41