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Titolo:
Forward and retrograde trafficking in mitotic animal cells - ER-Golgi transport arrest restricts protein export from the ER into COPII-coated structures
Autore:
Farmaki, T; Ponnambalam, S; Prescott, AR; Clausen, H; Tang, BL; Hong, WJ; Lucocq, JM;
Indirizzi:
Univ Dundee, Dept Anat & Physiol, Dundee DD1 4HN, Scotland Univ Dundee Dundee Scotland DD1 4HN & Physiol, Dundee DD1 4HN, Scotland Univ Dundee, Dept Biochem, Dundee DD1 4HN, Scotland Univ Dundee Dundee Scotland DD1 4HN pt Biochem, Dundee DD1 4HN, Scotland Fac Hlth Sci, Sch Dent, DK-2200 Copenhagen, Denmark Fac Hlth Sci Copenhagen Denmark DK-2200 ent, DK-2200 Copenhagen, Denmark Inst Mol & Cell Biol, Membrane Biol Lab, Singapore 117609, Singapore Inst Mol & Cell Biol Singapore Singapore 117609 gapore 117609, Singapore
Titolo Testata:
JOURNAL OF CELL SCIENCE
fascicolo: 5, volume: 112, anno: 1999,
pagine: 589 - 600
SICI:
0021-9533(199903)112:5<589:FARTIM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
VESICULAR STOMATITIS-VIRUS; EARLY SECRETORY PATHWAY; SYNTHESIZED G-PROTEIN; 58-KDA CIS-GOLGI; ENDOPLASMIC-RETICULUM; HELA-CELLS; INTERMEDIATE COMPARTMENT; LEGUMINOUS LECTINS; MEMBRANE-PROTEIN; VESICLE COATS;
Keywords:
mitosis; Golgi; COPII; budding compartment; recycling;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
53
Recensione:
Indirizzi per estratti:
Indirizzo: Lucocq, JM Univcotland, Dept Anat & Physiol, MSI WTB Complex,Dow St, Dundee DD1 4HN, S Univ Dundee MSI WTB Complex,Dow St Dundee Scotland DD1 4HN , S
Citazione:
T. Farmaki et al., "Forward and retrograde trafficking in mitotic animal cells - ER-Golgi transport arrest restricts protein export from the ER into COPII-coated structures", J CELL SCI, 112(5), 1999, pp. 589-600

Abstract

Protein transport arrest occurs between the ER and Golgi stack of mitotic animal cells, but the location of this block is unknown. In this report we use the recycling intermediate compartment protein ERGIC 53/p58 and the plasma membrane protein CD8 to establish the site of transport arrest, Recycled ERGIC 53/p58 and newly synthesised CD8 accumulate in ER cisternae but notin COPII-coated export structures or more distal sites. During mitosis thetubulovesicular ER-related export sites were depleted of the COPII component Sec13p, as shown by immunoelectron microscopy, indicating that COPII budding structures are the target for mitotic inhibition. The extent of recycling of Golgi stack residents was also investigated. In this study we used oligosaccharide modifications on CD8 trapped in the ER of mitotic cells as asensitive assay for recycling of Golgi stack enzymes. We find that modifications conferred by the Golgi stack-resident GalNac transferase do occur onnewly synthesised CD8, but these modifications are entirely due to newly synthesised transferase rather than to enzyme recycled from the Golgi stack. Taken together our findings establish for the first time that the site of ER-Golgi transport arrest of mitotic cells is COPII budding structures, andthey clearly speak against a role for recycling in partitioning of Golgi stack proteins via translocation to the ER.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 16:11:34