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Titolo:
Characterization of the proteases involved in gel weakening of beef heart surimi
Autore:
Wang, BW; Xiong, YLL;
Indirizzi:
Univ Kentucky, Dept Anim Sci, Lexington, KY 40546 USA Univ Kentucky Lexington KY USA 40546 pt Anim Sci, Lexington, KY 40546 USA
Titolo Testata:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
fascicolo: 3, volume: 47, anno: 1999,
pagine: 887 - 892
SICI:
0021-8561(199903)47:3<887:COTPII>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MACKEREL SCOMBER AUSTRALASICUS; CROAKER MICROPOGON-UNDULATUS; PACIFIC WHITING SURIMI; CATHEPSIN-L-LIKE; ALKALINE PROTEASE; ORDINARY MUSCLE; PURIFICATION; DEGRADATION; INHIBITOR; PROTEINS;
Keywords:
beef heart; surimi; cathepsin; protease; protease inhibitor;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Xiong, YLL Univ Kentucky, Dept Anim Sci, Lexington, KY 40546 USA Univ Kentucky Lexington KY USA 40546 , Lexington, KY 40546 USA
Citazione:
B.W. Wang e Y.L.L. Xiong, "Characterization of the proteases involved in gel weakening of beef heart surimi", J AGR FOOD, 47(3), 1999, pp. 887-892

Abstract

The goal of this study was to elucidate the nature and characteristics of the proteases involved in gel weakening of beef heart surimi. Acidic (El) and neutral (E2) protease extracts were prepared from the surimi. The major active components in El were found to be cathepsins B and L. El exhibited optimum activity to hydrolyze substrates specific to cathepsins B and B+L at50 degrees C and pH 5.5. At pH 6.0, it retained similar to 50% of its maximum activity. The catheptic activity of ET was inhibited almost completely by E-64 and leupeptin. The active component in E2 was unidentified and was not inhibited by cysteine or serine protease inhibitors. However, beef plasma powder effectively inhibited the hydrolysis of FITC-casein and myosin heavy chain by E2.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 20:24:54